TDZ促进花生外植体分化及基因转化  

TDZ Promote Differentiation and Gene Transformation of Peanut (Arachis hypogaea)

作　　者：梁丽琨[1] 林荣双[1] 肖显华[1] 王庆华[1] 

机构地区：[1]烟台大学生物化学系,山东烟台264005

出　　处：《烟台大学学报（自然科学与工程版）》2002年第3期189-194,共6页Journal of Yantai University(Natural Science and Engineering Edition)

基　　金：山东省科技厅资助项目 (SH95K1)

摘　　要：以花生成熟胚的上胚轴和胚叶为外植体 ,通过农杆菌介导转化Bt和CpTI双基因 ,在共培养基和诱导培养基中加入TDZ诱导不定芽和体细胞胚 .实验证明 ,TDZ有明显促进花生外植体分化的作用 ,当诱导培养基组成为TDZ 0 .3mg/L +NAA 0 .4mg/L ,诱导时间为 2 8d ,分化培养基为MS时采用TDZ发胚培养基萌发的轴外植体分化率最高 ,可达73% ,GUS基因阳性率 3.5 % .当诱导培养基组成为TDZ 0 .2mg/L +NAA 0 .4mg/L ,诱导时间为 2 1d ,分化培养基为MS时水萌发种胚叶外植体分化率最高 ,达 5 6 % ,GUS基因阳性率 2 .5 % .The epicotyls and leafs from mature peanut(Arahis hypogaea)seeds are inoculated with Agrobacterium tumefaciens strain LBA4404 separately harboring Bt and CpTI gene , and are cultivated on the medium containing TDZ for inducing somatic embryos and buds. The results show that TDZ can promote the differentiation of peanut .When the inducing medium supplied with TDZ 0.3 mg/L and NAA 0.4 mg/L,the inducing period is 28 d and the differentiation medium is MS ,the rate of differentiation of epicotyl expants is as high as 73%, and 3.5% of the germinated seedlings are GUS +. When the inducing medium supplied with TDZ 0.2 mg/L and NAA 0.4 mg/L, the inducing period is 21d and the differentiation medium is MS ,the rate of differentiation of leaf expants is as high as 56%, and 2.5% of the germinated seedlings are GUS +.

关 键 词：外植体 TDZ 花生 分化 基因转化 基因工程 不定芽 体细胞胚 细胞激动素 

分 类 号：S565.2[农业科学—作物学] Q943.2[生物学—植物学]