家鸡TSHβ、FSHβ和LHβ重组蛋白表达及纯化研究  

Expression and purification of recombinant chicken TSHβ,FSHβ,and LHβ proteins

作　　者：阎振鑫[1] 莫春横 王亚军[1] 李娟[1] 

机构地区：[1]四川大学生命科学学院生物资源与生态环境教育部重点实验室,成都610065

出　　处：《四川大学学报（自然科学版）》2016年第1期163-168,共6页Journal of Sichuan University(Natural Science Edition)

基　　金：国家自然科学基金(31172202)

摘　　要：本文以家鸡冷冻垂体组织为模版,克隆了TSHβ、亚基FSHβ亚基和LHβ亚基的部分特异性功能片段,并用pET原核表达系统构建了它们的原核表达质粒:pET32a-TSHβ、pET28a-FSHβ和pET32a-LHβ.利用大肠杆菌BL21(DE3)和Rosetta-gami TM菌株摸索到高效重组表达pET32a-TSHβ、pET28a-FSHβ和pET32a-LHβ的IPTG诱导浓度分别为:200μM、25μM和15μM.并在此基础上利用镍离子亲和层析蛋白纯化技术对重组蛋白进行了纯化.In this paper, part of special function DNAfragmentsof Thyroid-stimulating hormone （TSH） βsubunit, Follicle-stimulating hormone （FSH）β subunit and Luteinizing hormone （LH）β subunit were cloned fromfreezed chickenpituitary. And their prokaryotic express plasmids were constructed in pET system., pET32a-TSHβ, pET28a-FSHβ and pET32a-LHβ. The most effectiveconcentrationof Inductor, IPTG, is 200μM,25μM and 15μM for them , individually, in BL21（DE3）and Rosetta-gamiTM strain. Based on these work, the proteins were purified by nickel affinity chromatograph.

关 键 词：TSHβ FSHΒ LHβ 基因克隆 重组蛋白表达 蛋白纯化 

分 类 号：Q81[生物学—生物工程]