戊己丸方药配伍的对比  

Comparison of Wuji Wan Prescriptions

作　　者：高源[1] 靳凤云[2] 杨正奕 

机构地区：[1]贵州省食品药品检验所,贵阳550001 [2]贵阳中医学院,贵阳550025

出　　处：《中国实验方剂学杂志》2016年第1期27-31,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(30860364)

摘　　要：目的:研究戊己丸药味配伍的特征图谱及其化学成分的变化规律。方法:采用毛细管电泳(HPCE)与反相高效液相色谱法对不同拆方进行考察,毛细管电泳以50 mmol·L-1的硼砂缓冲液-甲醇(2∶1)缓冲液为背景电解质,工作电压25k V,压力进样5 k Pa×15 s,电解质封口5 k Pa×10 s,柱温15℃。反相高效液相色谱法采用Agelapromosil C18色谱柱(4.6 mm×250 mm,5μm),柱温25℃,流速0.8 m L·min-1,流动相乙腈(A)-0.05 mol·L-1磷酸二氢钾(含0.1%磷酸)(B)进行梯度洗脱,检测波长230 nm,进样量5μL。采用撤药分析法对戊己丸进行研究,从定性定量两个角度进行评价,从整体上考察不同配伍情况方剂中化学成分的变化。结果:HPCE法确定了9个特征指纹峰,HPLC法确定27个特征指纹峰,其中黄连为该方君药,其生物碱类成分在不同配伍中变化较大;白芍中成分变化较小。结论:运用特征图谱技术结合定性、定量方法研究复方配伍,对复方新剂型的研制起到一定的指导作用。Objective： To study the characteristic chromatogram of herbs in Wuji Wan and the change rules of the chemical components. Method： HPLC and HPCE method were used to study different separated prescriptions. The conditions for the capillary zone electrophoresis（ CZE） method were 50 mmol·L^-1Borax bufferMethanol（ 2∶ 1）,constant voltage of 25 k V,pressure sampling under 5 k Pa × 15 s,electrolyte sealing under5 k Pa × 10 s and column temperature at 15 ℃. Chromatographic experiments were performed on Agelapromosil C18column（ 4. 6 mm × 250 mm,5 μm）,with the mobile phase was acetomitrile（ A）-0. 05 mol·L^-1potassium dihydrogen phosphate（ including 0. 1% phosphoric acid）（ B） by a gradient elution; the detection wavelength was230 nm,column temperature was 25 ℃,the flow rate was 0. 8 m L·min^-1. The withdrawal analytical method was used for a qualitative and quantitative evaluation on Wuji Wan,in order to study the changes in chemical components in different prescriptions. Result： HPCE and HPLC were used to confirm 9 and 27 characteristic fingerprint peaks. Coptidis Rhizoma was the main herb of the prescriptions,with great changes in alkaloids in different prescriptions and small changes in Paeoniae Alba Radix. Conclusion： The fingerprint technology and the qualitative and quantitative methods are combined to guide the development of new compound prescriptions.

关 键 词：戊己丸 拆方研究 特征图谱 毛细管电泳 

分 类 号：R284.1[医药卫生—中药学]