HPLC同时测定复方双花咀嚼片中5个成分  

Simultaneous Determination of Five Effective Components in Fufang Shuanghua Jujuepian by HPLC

作　　者：谭高好 杜莹[2] 乙引[3] 田红红[3] 杨建华 郭丽[3] 伍庆[1] 

机构地区：[1]贵州师范大学山地环境重点实验室,贵阳550001 [2]贵州师范大学化学与材料科学学院,贵阳550001 [3]贵州师范大学生命科学学院,贵阳550001 [4]贵州康纳圣方药业有限公司,贵阳550004

出　　处：《中国实验方剂学杂志》2016年第1期40-43,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：贵阳市科技计划项目(筑科合同[2012101]12号;黔教合协同创新字[2014]04)

摘　　要：目的：建立RP-HPLC同时测定复方双花咀嚼片中绿原酸、咖啡酸、连翘苷、穿心莲内酯和脱水穿心莲内酯的方法。方法：采用RP-HPLC法,依利特C18色谱柱（4.6 mm×250 mm,5μm）;流动相乙腈（A）-0.2%甲酸（B）,梯度洗脱（0~8 min,4%~13%A;8~24 min,13%~14%A;24~25 min,14%~26%A;25~65 min,26%~33%A;65~70 min,33%~50%A）,流速1 m L·min-1,检测波长240 nm,柱温30℃。结果：绿原酸、咖啡酸、连翘苷、穿心莲内酯和脱水穿心莲内酯分别在0.282 5~2.825 0μg（r=0.999 5）,0.086 8~0.867 5μg（r=0.999 7）,0.480 0~4.800 0μg（r=0.999 5）,0.158 8~1.587 5μg（r=0.999 5）,0.148 8~1.487 5μg（r=0.999 4）呈良好的线性关系,平均回收率分别为97.3%,RSD 1.2%;97.8%,RSD 1.1%;98.9%,RSD 1.4%;97.6%,RSD 1.0%;99.0%,RSD 1.5%。结论：该方法简洁、可靠、专属性强,可用于复方双花咀嚼片的质量控制。Objective： To establish a RP-HPLC method for simultaneously determining the contents of chlorogenic acid, caffeic acid forsythin, andrographalide and dehydroandrographalide in Fufang Shuanghua Jujuepian. Method： RP-HPLC was carried out on a hypersil C18（ 4. 6 mm × 250 mm,5 μm）. The mobile phase was A（ acetonitrile）-B（ 0. 2% formic acid） in the gradient elution mode（ 0-8 min,4%-13% A; 8-24 min,13%-14% A; 24-25 min,14%-26% A; 25-65 min,26%-33% A; 65-70 min,33%-50% A）. The flow rate was1. 0 m L·min- 1and the detection wavelength was set at 240 nm. The column temperature was maintained at 30 ℃.Result： The calibration curves of chlorogenic acid, caffeic acid, forsythin, andrographolide and dehydroandrographolide were in good linearity within the range of 0. 282 5-2. 825 0 μg（ r = 0. 999 5）,0. 086 8-0. 867 5 μg（ r = 0. 999 7）,0. 480 0-4. 800 0 μg（ r = 0. 999 5）,0. 158 8-1. 587 5 μg（ r = 0. 999 5）,0. 148 8-1. 487 5 μg（ r = 0. 999 4） and the average recoveries were 97. 3%（ RSD of 1. 2%）,97. 8%（ RSD of 1. 1%）,98. 9%（ RSD of 1. 4%）,97. 6%（ RSD of 1. 0%）,99. 0%（ RSD of 1. 5%）. Conclusion： The method is simple,reliable and specific,and can be applied for quality control of Fufang Shuanghua Jujuepian.

关 键 词：复方双花咀嚼片 绿原酸 咖啡酸 连翘苷 穿心莲内酯 脱水穿心莲内酯 

分 类 号：R284.1[医药卫生—中药学]