大承气汤主要成分在急性胰腺炎大鼠的组织分布特征  

Tissue Distribution of Main Components from Dachengqi Tang in Acute Pancreatitis Rats

作　　者：李君仪 竹林[1] 向瑾[1] 赵健蕾[1] 李娟[1] 郭徽[1] 唐文富[1] 

机构地区：[1]四川大学华西临床医学院/华西医院,华西基础医学与法医学院,成都610041

出　　处：《中国实验方剂学杂志》2016年第1期82-87,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81374042,1370091)

摘　　要：目的:研究大承气汤主要成分在急性胰腺炎(acute pancreatitis,AP)大鼠体内的组织分布特征,揭示靶器官分布的潜在药效成分。方法:雄性SD大鼠随机分为假手术组、模型组、正常给药组(正常组)和AP给药组(AP组),用3.5%牛磺胆酸钠逆行胰胆管注射建立AP模型,造模后4 h以大承气汤按10 g·kg-1灌胃1次,24 h后处死取血浆和器官,利用高效液相色谱-质谱/质谱(HPLC-MS/MS)分析各成分在血浆及各器官组织分布情况及对各组织血液的穿透率(penetration ratio,PR)。结果:大承气汤中大黄酸、和厚朴酚[(105.5±57.8),(40.8±6.7)μg·L-1]在AP组大鼠胰腺组织的质量浓度高于正常组[(31.9±19.4),(14.5±10.4)μg·L-1](P<0.05);AP组大承气汤主要单体对各组织的PR小于正常组。结论:AP状态可降低大承气汤中主要成分在各组织内的浓度;大黄酸、和厚朴酚在AP状态下有胰腺组织特异性分布特点,可能是大承气汤治疗AP大鼠对胰腺起靶向治疗作用的潜在药效成分,为研究该方剂治疗AP的机制提供线索。Objective： To explore tissue distribution of major constitutes form Dachengqi Tang in acute pancreatitis（ AP） rats. Method： Male SD rats were randomly divided into shamp operation group,model group,normal administration group and AP administration group. AP model was established with 3. 5% sodium taurocholate retrograding cholangiopancreatography injection. Rats were orally administered Dachengqi Tang with10 g·kg^-1after 4 hours following model being established. Heart,liver,spleen,lung,kidney,large intestine,pancreas and blood were then collected 24 hours later. Components and their distribution in various tissues and blood plasma were detected by HPLC-MS / MS,and penetration ratio（ PR） was analyzed respectively. Result：Concentrations of rhein and honokiol [（ 105. 5 ± 57. 8）,（ 40. 8 ± 6. 7） μg·L^-1,respectively]in the pancreas of AP rats was higher than that of the normal group [（ 31. 9 ± 19. 4）,（ 14. 5 ± 10. 4） μg·L^-1,respectively].Compared to normal rats,PR of blood-to-tissue of main components in AP group was less than that in the normal group. Conclusion： Tissue distribution of main components in Dachengqi Tang are inhibited under AP condition,except for rhein and honokiol, which shows these two components targeted distribution in pancreas might be potential pharmacological substances of Dachengqi Tang for AP.

关 键 词：急性胰腺炎 大承气汤 器官靶向分布 大黄酸 和厚朴酚 大黄素 

分 类 号：R969.1[医药卫生—药理学] R945[医药卫生—药学]