十五味乳鹏散对尿酸钠致大鼠急性痛风性膝关节炎COX-2,PGE_2水平的影响  

Influence of Rupeng 15 Powder on COX-2,PGE_2 Protein and mRNA Expression Levels in Rat with Mono Sodium Urate Crystals-induced Gouty Arthritis

作　　者：寇毅英[1] 李永芳[1] 杨梅[1] 吕慧玲[1] 李瑞莲[1] 

机构地区：[1]青海大学医学院,西宁810001

出　　处：《中国实验方剂学杂志》2016年第1期117-120,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81160410)

摘　　要：目的:通过观察十五味乳鹏散对尿酸钠(MSU)致急性痛风性关节炎大鼠膝关节液及滑膜组织中环氧酶2(COX-2),前列腺素E2(PGE2)蛋白及mRNA水平的影响,探讨十五味乳鹏散治疗急性痛风性关节炎的机制。方法:大鼠分为正常组,模型组,十五味乳鹏散低、中、高剂量(0.4,0.8,1.2 g·kg-1)组,吲哚美辛(3 mg·kg-1)组。正常组和模型组ig生理盐水;各给药组按剂量ig给药,连续7 d。末次给药1 h后,正常组双侧膝关节腔注射50μL无菌1%磷酸盐缓冲液(PBS),其余各组注射50μL尿酸钠溶液,于造模后24 h用酶联免疫吸附试验(ELISA)和实时荧光定量PCR(QPCR)检测关节滑液和滑膜组织COX-2,PGE2蛋白及mRNA水平。结果:与正常组比较,模型组MSU注射进大鼠膝关节腔24 h后,COX-2,PGE2在大鼠关节滑液中的含量及在滑膜组织中的mRNA表达量均明显增加(P<0.01);与模型组比较,十五味乳鹏散低、中、高剂量组及吲哚美辛组均显著降低MSU致大鼠痛风性关节炎关节滑液COX-2和PGE2的蛋白水平及滑膜组织COX-2 mRNA水平(P<0.05,P<0.01),但滑膜组织PGE2mRNA水平在各组间并无显著性差异。结论:MSU可以增加关节滑液及滑膜组织中COX-2的表达,同时伴有COX-2,PGE2水平的增加;十五味乳鹏散可能通过降低COX-2的表达,降低COX-2,PGE2的水平产生抗MSU痛风性关节炎的作用。Objective： To observe the effects of Rupeng 15 powder（ RPP15） on cyclooxygenase-2（ COX-2）,prostaglandin E2（ PGE2） protein and mRNA expression levels in the knee joint fluid and synovium tissues,and explore the therapeutic mechanism of RPP15 in rats with monosodiu murate（ MSU） crystals-induced gouty arthritis. Method： The rats were dividied into normal group,model group,RPP15 low-dose,middle-dose and high dose groups（ 0. 4,0. 8,1. 2 g·kg^-1）,indomethacin group（ 3 mg·kg^-1）. The normal group and model group were ig administered with normal saline; various treatment groups were ig administered with corresponding doses for continuous 7 days. One hour after the last administering,50 μL sterile 1% PBS was injected in bilateral knee joint cavity in normal group,and 50 μL MSU was injected in other groups. Twenty-four h after modeling,enzyme linked immunosorbant assay（ ELISA） method and real-time fluorescent quantitative PCR method were used to detect COX-2,PGE2 protein and mRNA expression levels in the knee joint fluid and synovium tissues. Result：Data from our study showed that rat with gouty arthritis induced by MSU crystal demonstrated an elevation in COX-2,PGE2 in knee joint fluid and mRNA expression levels in synovium tissues（ P〈0. 01）. Compared with the model group,RPP15（ 0. 4,0. 8,1. 2 g·kg^-1） groups and indomethacin group had significantly lower COX-2 and PGE2 protein levels in knee joint fluid COX-2 mRNA level in synovium tissues（ P〈0. 05,P〈0. 01）,but PGE2 mRNA was no statistically significant difference in synovium tissues. Conclusion： MSU can increase the expression of COX-2 and PGE2 in knee joint fluid and synovium tissues. RPP15 may have the effect on MSUinduced gouty arthritis by decreasing COX-2 expression,COX-2 level and PGE2 level.

关 键 词：十五味乳鹏散 尿酸钠 痛风性关节炎 

分 类 号：R285.5[医药卫生—中药学]