桃仁提取物抗血瘀证大鼠血栓形成的机制  

Antithrombotic Mechanism of Persicae Semen Extract in Blood Stasis Rats

作　　者：以敏[1,2] 徐君毅[2] 郝二伟[3] 邓家刚[3] 

机构地区：[1]广西中医药大学第一附属医院,南宁530023 [2]广西医科大学第四附属医院,广西柳州545001 [3]广西中医药大学重点实验室,南宁530001

出　　处：《中国实验方剂学杂志》2016年第1期125-128,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家重点基础研究发展计划(973计划)项目(2007CB512602);广西自然科学基金项目(2014NSFBA118200);广西卫生厅自筹课题(Z2011112)

摘　　要：目的:观察桃仁提取物对瘀热互结证大鼠抗微血栓效应,检测其与血小板黏附分子31(CD31),核转录因子-κB(NF-κB)及其抑制物κB(IκB)表达的关系,探讨桃仁对血瘀证抗血栓形成的机制。方法:60只SD大鼠随机分为正常组,模型组,桃仁提取物低、中、高剂量组(2,4,8 g·kg-1)及丹参组(14 g·kg-1),用角叉菜胶+干酵母复合注射法建立瘀热互结证模型,用药组分别按剂量ig给药,连续7 d。观察各组血瘀体征,全血黏度,血浆黏度及纤维蛋白含量;组织病理观察微血栓和肾损伤;免疫组化,原位杂交检测分析内皮细胞CD31,NF-κB,IκB蛋白及mRNA表达吸光度A。结果:与正常组比较,模型组大鼠出现血瘀体征:全身皮肤黏膜充血,肿胀,发绀;全血黏度,微血栓,肾损伤增多或明显;内皮CD31蛋白,NF-κB核蛋白,IκB总蛋白增多,NF-κB总蛋白显著减少(P<0.01)。与模型组比较,桃仁提取物高剂量组血瘀体征减轻,全血黏度,血浆黏度,微血栓及肾损伤均明显降低或减少(P<0.01),内皮CD31蛋白减少,NF-κB,IκB总蛋白及mRNA均明显增多(P<0.05,P<0.01)。结论:桃仁提取物能显著改善瘀热互结证,降低血黏度,抑制微血栓形成,保护肾实质;其可能通过降低CD31表达,促进NF-κB及其抑制物IκB的基因转录和翻译,或抑制其降解,从而发挥抗微血栓的作用。Objective： To study the antithrombotic effect of Persicae Semen extract（ PSE） in blood stasis rats,its relationship with platelet adhesion molecule 31（ CD31）,nuclear transcription factor-κB（ NF-κB）and its inhibitor κB（ IκB） expressions and the antithrombotic mechanism of Semen Persicae against blood stasis syndrome. Method： Sixty SD rats were randomly divided into the normal group,the model group,the PSE low-,middle-,high-dose groups（ 2,4,8 g·kg^（-1）） and Danshen group（ 14 g·kg^（-1））. The stasis model was established through ip with carrageenan and dry yeast liquid. All of treatment groups were given drugs by oral administration.The stasis symptom,blood viscosity,plasma viscosity,fibrinogen,micro-thrombosis and renal damage were observed by blood viscometer and histopathological method. CD31,NF-κB,IκB protein and mRNA expression absorbance of vascular endothelial cells were detected by immunohistochemistry method and in situ hybridization technique. Result： Compared with the normal group,the model group had some stasis symptoms,such as congestion,swelling, cyanosis under skin mucosa; its blood viscosity, micro-thrombosis and renal injury increased,CD31,nuclear NF-κB and total-IκB protein of endothelial increased,but NF-κB total protein decreased（ P〈0. 01）. Compared with the model group,the PSE high dose group showed relieved stasis symptoms,lower blood viscosity,plasma viscosity,micro-thrombosis and relieved kidney damage（ P〈0. 01）,decreased CD31 protein of endothelial,but higher NF-κB,IκB total protein and mRNA（ P〈0. 05,P〈0. 01）. Conclusion： PSE can significantly improve blood stasis symptoms,lower blood viscosity,inhibit thrombosis and protect renal. It might show the anti-micro thrombosis effect by promoting NF-κB and its inhibitor IκB gene transcription and translation,or inhibiting its degradation,as well as reducing the CD31 protein.

关 键 词：桃仁 血瘀证 血管内皮 血小板黏附分子31 核转录因子-ΚB 

分 类 号：R285.5[医药卫生—中药学]