丹龙醒脑方对脑缺血再灌注大鼠血管新生及对Ang-1蛋白表达的影响  

Effect of Danlong Xingnao Decoction on Angiogenesis and Ang-1 Protein Expressions in Rats with Cerebral Ischemia-reperfusion

作　　者：张秋雁[1] 朱伟[1] 徐瑾瑜 唐金强[1] 梁昊[1] 苏剑峰[1] 唐映红[1] 逯晶[1] 

机构地区：[1]湖南中医药大学,长沙410208 [2]华恩堂养生管理有限公司,长沙410000

出　　处：《中国实验方剂学杂志》2016年第1期139-142,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：湖南省高校创新平台开放基金项目(12K090)

摘　　要：目的:研究丹龙醒脑方对脑缺血再灌注大鼠血管新生及血管生成素-1(Ang-1)的影响。方法:用线栓法制备大鼠大脑中动脉栓塞缺血再灌注(MCAO)模型,2 h后开放血流再灌注。大鼠造模成功后被分为6组(丹龙醒脑方14.76,7.38,3.69 g·kg-1剂量组,尼莫地平10.8 mg·kg-1组,模型组,假手术组),ig 7 d后处死大鼠取脑,用免疫组化法测定缺血海马区的内皮细胞数和微血管密度(MVD)及Ang-1的蛋白表达。结果:与假手术组比较,模型组大鼠内皮细胞计数及MVD,Ang-1蛋白表达均明显增强(P<0.05);与模型组相比,丹龙醒脑方组内皮细胞数、MVD及Ang-1蛋白表达均有明显升高(P<0.05,P<0.01)。结论:丹龙醒脑方能够促进脑缺血再灌注大鼠缺血脑组织血管新生,对缺血脑组织具有保护作用,其作用机制可能与上调Ang-1蛋白表达有关。Objective： To investigate the effect of Danlong Xingnao decoction on the angiogenesis and the angiopoitin 1（ Ang-1） protein expression of rats with cerebral ischemia-reperfusion. Method： A rat model of the middle cerebral artery occlusion / reperfusion（ MCAO） was created by using the suture method,and the blood reperfusion was performed 2 hours later. The rats were randomly divided into six groups,high-dose,medium-dose and low-dose（ 14. 76,7. 38,3. 69 g·kg^-1） Danlong Xingnao decoction groups,nimodipine（ 10. 8 mg·kg^-1）group,model group and sham group. The rats were given by gavage once a day for 7 days. The microvessel vascular density（ MVD） were observed and the expression of Ang-1 at protein level were studied by the immuneohistochemical method. Result： Compared with the sham group,the microvessel density,Ang-1 in model group were higher significantly（ P〈0. 05）. Compared with the model group,Danlong Xingnao decoction groups showed significantly higher levels of MVD and the protein expression of Ang-1（ P〈0. 05, P〈0. 01）.Conclusion： Danlong Xingnao decoction could promote angiogenesis and protect ischemic brain tissue in rats. Its mechanism may be correlated with the up-regulation of the expression of Ang-1.

关 键 词：丹龙醒脑片 脑缺血再灌注 血管新生 血管生成素-1 

分 类 号：R285.5[医药卫生—中药学]