不同MTHFR基因型子痫前期患者感染病原菌对血清β-HCG与和PLGF水平的影响研究  

Influence of pathogens causing infections on levels of serumβ-HCG and PLGF of preeclampsia patients with different MTHFR genotypes

作　　者：刘湘萍[1] 杨晓文[1] 黄婷[1] 王竞[1] 叶麒[1] 

机构地区：[1]萍乡市人民医院产科,江西萍乡337000

出　　处：《中华医院感染学杂志》2016年第1期150-152,共3页Chinese Journal of Nosocomiology

基　　金：江西省卫生厅基金资助项目(2012YDS18152)

摘　　要：目的研究不同亚甲基四氢叶酸还原酶(MTHFR)基因型子痫前期患者感染病原菌分布,并探讨其对患者血清人绒毛膜促性腺激素(β-HCG)和胎盘生长因子(PLGF)水平的影响,为临床治疗提供参考。方法对医院2010年1月-2015年6月诊治的不同子痫感染患者采用BD PHOENIXTM全自动细菌鉴定仪对病原菌进行鉴定,酶联免疫吸附测定法分析感染后患者血清中β-HCG和PLGF水平的变化;以医院同期健康孕妇120例为对照组,子痫前期感染患者120例为感染组;子痫前期未感染患者140例为未感染组;比较3组患者MTHFR基因C667T多态分布率及血清中β-HCG和PLGF变化;采用WHONE5.5软件和SPSS20.0软件进行统计分析。结果子痫前期患者C667T基因T/T频率明显增高,而C/T基因频率明显降低,且与对照组比较差异有统计学意义(P<0.05);而C/C、T/T和C/T基因型感染患者病原菌种类和构成比差异有统计学意义,且子痫感染组患者血清中β-HCG和TNFα及IL6蛋白水平明显升高而PLGF水平明显降低(P<0.01)。结论不同MTHFR基因型子痫前期患者感染病原菌无明显差异,且感染后患者血清β-HCG水平明显升高而PLGF明显降低。OBJECTIVE To investigate the distribution of pathogens causing infections in the preeclampsia patients with different methylenetetrahydrofolate reductase（MTHFR）genotypes and explore the impact on the levels of serum human chorionic gonadotropin（β-HCG）and placental growth factor（PLGF）so as to provide guidance for clinical treatment.METHODS From Jan 2010 to Jun 2015,the pathogens isolated from the patients with different eclampsia infection were identified by using BD PHOENIXTM automatic bacteria identification system,the levels of serumβ-HCG and PLGF of the patients with infections were determined by using enzyme-linked immunosorbent assay.During the same period,totally 120 healthy pregnant women were chosen as the control group,120 preeclampsia patients with infections were assigned as the infection group,and 140 preeclampsia patients without infections were assigned as the non-infection group.The polymorphism distribution of the MTHFR gene C667 Tand the levels of serumβ-HCG and PLGF were observed and compared among the three groups of patients,and the statistical analysis was performed with the use of WHONET5.5software and SPSS20.0software.RESULTS The frequency of the C667 Tgene T/T was significantly increased in the preeclampsia patients,while the frequency of the C/T gene was significantly decreased,as compared with the control group（P〈0.05）.There was significant difference in the species or constituent ratios of the pathogens among the infection patients with the C/C,T/T,and C/T genotypes;the levels of serumβ-HCG,TNFα,and IL6 of the preeclampsia patients with infections were significantly elevated,while the PLGF level was remarkably decreased（P〈0.01）.CONCLUSIONThe preeclampsia patients with different MTHFR genotypes do not vary significantly in the distribution of pathogens causing infections.The serumβ-HCG level of the patients with infections is significantly elevated,while the PLGF level is reduced.

关 键 词：亚甲基四氢叶酸还原酶 基因型 子痫前期 病原菌 

分 类 号：R378[医药卫生—病原生物学]