广西HIV-1流行株env基因C2V3区序列特征和亚型研究  

Subtype and sequence analysis of C2V3 region of env gene among HIV-1 strains in Guangxi

作　　者：王敏连 梁冰玉[1] 叶力[1] 潘沛江 陈荣凤[1] 赵芳凝 蒋俊俊[1] 黄颉刚[1] 王洪[1] 刘洁[1] 周波[1] 梁浩[1] 

机构地区：[1]广西医科大学公共卫生学院流行病与卫生统计学教研室,广西艾滋病防治研究重点实验室,广西南宁530021

出　　处：《中华疾病控制杂志》2015年第12期1191-1194,共4页Chinese Journal of Disease Control & Prevention

基　　金：国家自然科学基金(81171624;31360033;81360259);广西自然科学基金(2013GXNSFBA01966);广西医科大学青年基金(GXMUYSF201344)

摘　　要：目的研究广西艾滋病病毒1型流行株的亚型及env基因C2V3区序列变异特征。方法 2009-2012年共收集138份广西HIV-1感染者血浆样本,提取病毒RNA,用巢式聚合酶链反应对env基因C2V3区序列进行扩增,并测序,用MEGA、Hy Phy等软件对序列信息进行分析。结果 138份样本中,CRF01_AE亚型72例;CRF08_BC亚型40例;B亚型13例;CRF07_BC亚型8例;C亚型2例。各亚型基因离散率分别为:CRF01_AE(0.052±0.053);CRF08_BC(0.044±0.048);CRF07_BC(0.030±0.041);B亚型(0.024±0.044)。各亚型之间基因离散率差异无统计学意义(F=1.260,P=0.291)。CRF01_AE的负向选择压力比CRF08_BC大(t=10.060,P<0.001)。共发现7种V3环顶端四肽,其中CRF01_AE有6种,CRF08_BC亚型有2种,两者均以GPGQ为主;B(B’)亚型有2种,以GPGR为主,CRF07_BC仅出现了1种。在CRF01_AE亚型和B亚型中分别发现了新的V3环顶端四肽GPGM和GTGR。各亚型辅助受体均主要为CCR5。结论 CRF01_AE亚型在广西HIV-1流行株中占优势,其V3环顶端四肽以GPGQ为主。CRF01_AE亚型和B亚型V3环序列出现了新的变异特征。广西HIV-1流行株主要为巨噬细胞嗜性的非合胞体诱导型。Objective To investigate the subtype distribution and sequence characteristics of C2V3 region of env gene among HIV-1 strains prevalent in Guangxi. Methods Plasma samples were collected from 138 HIV-1 seropositive individuals during 2009- 2012 and were used for HIV-1 RNA extraction. The C2V3 region of env gene was amplified by nested polymerase chain reaction and then was sequenced. Analysis of the sequences was performed by softwares MEGA and Hy Phy. Results Of the 138 samples,there were 72 cases of CRF01_AE subtype,40 cases of CRF08_BC,13 cases of subtype B,8 cases of CRF07_ BC and 2 cases of subtype C. The innergroup gene divergences of subtype CRF01_ AE,CRF08_BC,CRF07_BC,and B were（ 0. 052 ± 0. 053）,（ 0. 044 ± 0. 048）,（ 0. 030 ± 0. 041） and（ 0. 024 ± 0. 044）,respectively,no significant differences were found among the four groups（ F = 1. 260,P = 0. 291）. The negative selection pressure of CRF01_AE was significantly higher than that of CRF08_BC（ t = 10. 060,P〈0. 001）. Seven types of V3-tip motifs were found in this investigation,with 6 types existing in subtype CRF01_AE,2 in CRF08_BC,2 in B（ B＇）,and1 in CRF07_BC. In subtypes CRF01_AE and CRF08_BC,GPGQ was the predominant type,while in subtype B（ B＇）,GPGR was the predominant one. Two new types of V3-tip motifs,GPGM and GTGR,were identified in subtype CRF01_AE and B,respectively. The main co-receptor of all subtypes was CCR5. Conclusions CRF01_AE was the predominant HIV-1 subtype among HIV-1 strains prevalent in Guangxi,with GPGQ as the dominant type of V3-tip motif. Two new types of V3-tip motifs were identified in subtype CRF01_AE and B. Most of HIV-1 strains in Guangxi belong to macrophage-tropic and non-syncytium-inducing HIV-1.

关 键 词：HIV-1 基因 env 变异(遗传学) 

分 类 号：R512.9[医药卫生—内科学]