小鼠卵母细胞和胚胎发育过程中组蛋白H4K20二甲基化的表达  

Expression of histone H4K20 dimethylation during the development of mouse oocyte and embryo

作　　者：时小艳[1] 刘庆新[2] 

机构地区：[1]江苏食品药品职业技术学院医药与健康管理系,淮安223003 [2]江苏农林职业技术学院,句容212400

出　　处：《上海农业学报》2015年第6期45-48,共4页Acta Agriculturae Shanghai

基　　金：国家"973"重点基础研究发展计划项目(2007CB947403)

摘　　要：运用单细胞免疫荧光技术,分析了小鼠卵母细胞和胚胎发育过程中组蛋白H4K20二甲基化的表达情况。结果表明:H4K20二甲基化在生长期的卵母细胞中呈逐渐增加的趋势;15 d时的小鼠卵母细胞没有H4K20二甲基化的表达,但在第20天和第25天重新被检测到信号;卵母细胞在生发泡期(GV)、生发泡破裂期(GVBD)和减数分裂中期(MⅡ)均没有H4K20二甲基化的表达;二细胞胚胎和四细胞胚胎中有H4K20二甲基化的表达,其他时期的胚胎中检测不到荧光信号。The expression of histone H4K20 dimethylation during the development of mouse oocyte and embryo was analysed by single cell immunofluorescence technique.The results showed that H4K20 dimethylation gradually increased during the growth period of oocytes;there was no H4K20 dimethylation expression in the 15 th day mouse oocytes,but the expression signal was detected again in the 20 th and 25 th day mouse oocytes.There was no H4K20 dimethylation expression in oocytes during the period of GV,GVBD and M II.H4K20 dimethylation expressed in two cell embryos and four cell embryos,but the fluorescence signal was not detected in other periods of embryo.

关 键 词：卵母细胞 胚胎 H4K20 表达 

分 类 号：S512.1[农业科学—作物学]