中华鳖和中华草龟的过敏原组分分析  

Study on Allergens of T. Sinensis and C. reevesii

作　　者：毛露甜[1] 马伟兵 

机构地区：[1]惠州学院生命科学系,广东惠州516007

出　　处：《惠州学院学报》2015年第6期20-22,共3页Journal of Huizhou University

基　　金：惠州市科技计划资助项目(2006P45)

摘　　要：采用聚丙烯酰胺凝胶电泳（SDS-PAGE）和免疫印迹法（Western blot）分析中华鳖和中华草龟的蛋白组分和主要过敏原。结果表明,中华鳖有8个蛋白组分,相对分子质量（Mr）分布在25 000~110 000之间,主要过敏原在42 000和64 000附近,中华草龟有10个蛋白组分,Mr分布在25 000~70 000之间,主要过敏原组分Mr在36 000附近。提示中华鳖和草龟有相近的蛋白条带,在36 000和42 000之间可能有共同的过敏原,但是否存在交叉反应有待进一步验证。The main allergens of T. Sinensis and C. reevesii were analyzed by SDS-PAGE and Westernblot assay. Results show that T.Sinensis have 8 protein components, with the relative molecule mass（Mr） between 25000 and 110000, the main allergic protein close to42 000 and 64 000; that C. reevesii have 10 protein components, with the Mr between 25000 and 70000, the main allergic protein close to 36 000. This suggests that T. Sinensis and C. reevesii have similar protein band, and there may be common allergens between 36 000-42 000 in T. Sinensis and C. reevesii perheply, but whether cross reaction exists needs further confirmation.

关 键 词：中华鳖 中华草龟 过敏原 免疫印迹 

分 类 号：S144[农业科学—肥料学]