牙周膜干细胞分化、增殖特性与牙周组织再生  

Periodontal ligament stem cell differentiation and proliferation in periodontal tissue regeneration

作　　者：朱凌兰[1] 许生敏[2] 

机构地区：[1]武警江苏总队医院南京分院口腔科,江苏省南京市210028 [2]武警江苏总队医院南京分院急诊科,江苏省南京市210028

出　　处：《中国组织工程研究》2015年第45期7369-7373,共5页Chinese Journal of Tissue Engineering Research

摘　　要：背景:牙周膜干细胞是牙周组织再生工程中理想的种子细胞之一,有关牙周膜干细胞的来源、生物学特性及影响因素等研究成为热点问题。目的:就近年来牙周膜干细胞的研究现状、生物学特性、功能影响因素及其在再生医学中的应用等研究进展作一综述,并对其应用前景及目前存在的问题进行讨论,为相关体内研究提供理论和实验依据。方法:由第一作者在Pub Med和万方数据库检索2002至2015年有关牙周膜干细胞分化、增殖特性的相关英文和中文文献。中文检索词为"牙周膜干细胞,牙周组织,增殖,分化",英文检索词为"periodontal ligament stem cell,periodontal tissue,proliferation,differentiation",最终纳入47篇文献。结果与结论:牙周膜干细胞具有成纤维、成脂、成骨、成牙骨质分化的能力,细胞生长因子对牙周膜干细胞的增殖分化起重要作用,成纤维细胞生长因子、血管内皮生长因子和胰岛素样生长因子均可促进牙周膜干细胞增殖。干细胞载体生物膜材料的应用也能有效引导牙周组织再生,牙周膜干细胞的分离培养及其影响因素等还有待进一步完善,将干细胞与生物膜材料、生长因子联合应用达到理想的牙周组织功能性再生是今后的研究重点。BACKGROUND： Periodontal ligament stem cells are one of the ideal seed cells in periodontal tissue regeneration. Sources, biological characteristics and influential factors of periodontal ligament stem cells have been an issue of concern.OBJECTIVE： To review the biological characteristics and functional factors of periodontal ligament stem cells as well as relevant research status and progress in regenerative medicine, and to discuss the relevant application prospect and existing problems, thereby providing theoretical and experimental basis. METHODS： PubMed and Wanfang databases were searched by the first author for articles related to differentiation and proliferation of periodontal ligament stem cells published from 2002 to 2015. The key words were ＂periodontal ligament stem cell, periodontal tissue, proliferation, differentiation＂ in English and Chinese, respectively. Finally, 47 articles were included in result analysis. RESULTS AND CONCLUSION： Periodontal ligament stem cells have the ability of differentiating into fibroblasts, adipocytes, chondrocytes and cementum. Cell growth factors play an important role in the proliferation and differentiation of periodontal ligament stem cells. Fibroblast growth factor, vascular endothelial growth factor and insulin-like growth factor can promote the proliferation of periodontal ligament stem cells. Application of stem cell biofilm as a carrier material can effectively guide periodontal tissue regeneration. Isolation, culture and influential factors of periodontal ligament stem cells should be further improved. Combined application of stem cells, biofilms and growth factors is expected to achieve the desired periodontal tissue regeneration, which is the focus of future research.

关 键 词：干细胞 牙周膜 细胞增殖 引导组织再生 牙周 组织工程 分化 牙源性干细胞 牙周膜干细胞 增殖 牙周再生 

分 类 号：R318[医药卫生—生物医学工程]