正畸牙移动过程中尼古丁摄入对牙周组织影响  

Effect of nicotine intake on the periodontal tissue during orthodontic tooth movement

作　　者：潘旭[1,2] 祖丽呼玛.阿热甫江 胡明华[1,2] 聂晶[1,2] 米丛波[1,2] 杨凤莲 

机构地区：[1]新疆医科大学第一附属医院口腔正畸科,新疆维吾尔自治区乌鲁木齐市830054 [2]新疆医科大学口腔医学院口腔正畸教研室,新疆维吾尔自治区乌鲁木齐市830054 [3]新疆库尔勒市农二师医院综合门诊部口腔科,新疆维吾尔自治区库尔勒市841000

出　　处：《中国组织工程研究》2015年第46期7406-7412,共7页Chinese Journal of Tissue Engineering Research

基　　金：新疆医学动物模型研究实验室开放课题基金资助(XJDX1103-2012-04);课题名称:尼古丁对正畸力作用下大鼠牙周组织改建的影响~~

摘　　要：背景:吸烟是严重影响牙周组织和牙根健康的危险因素之一,烟草中的尼古丁会加速牙周病患者牙周组织的破坏。目的:分析正畸牙移动过程中不同剂量尼古丁作用下牙周组织内环氧化酶2及mR NA表达的变化规律。方法:将110只SD大鼠随机分为5组,空白对照组10只,生理盐水组和尼古丁0.5,0.75,1 mg/kg组各25只。除空白组外所有大鼠使用50 g力牵拉一侧上颌第1磨牙向近中移动。生理盐水组每日腹腔注射0.1 mL生理盐水;尼古丁组每日腹腔注射0.5,0.75,1 mg/kg尼古丁酒石酸溶液。各组大鼠分别在加力1,3,5,7,14 d时处死取上颌组织,苏木精-伊红染色观察牙周组织变化,免疫组织化学染色计数破牙骨质细胞阳性细胞,原位杂交染色法检测环氧化酶2在牙周组织中表达的平均吸光度值。结果与结论:尼古丁各剂量组在各加力时间点破牙骨质细胞数目均高于未注射尼古丁组,且环氧化酶2阳性细胞表达强度也均高于未注射尼古丁组。随尼古丁注射剂量增大,破牙骨质细胞数目也逐渐增加(P<0.05),加力7 d时各组破牙骨质细胞数目均达到峰值;环氧化酶2阳性细胞表达强度也随尼古丁注射剂量增大而增强(P<0.05),加力5 d时表达强度达到峰值。结果表明,在相同加力时间点,破牙骨质细胞数目随剂量增大而增多,且环氧化酶2阳性细胞表达强度也随剂量增加而增强。正畸牙移动过程中的尼古丁摄入会造成牙周组织的破环,且尼古丁摄入剂量的高低直接影响牙周组织的破坏程度。BACKGROUND：Cigarette smoking can seriously damage the periodontal tissues and root, and the nicotine in tobacco accelerates the progression of periodontal diseases. OBJECTIVE： To investigate the effect of different doses of nicotine on the expression of cyclooxygenase-2 mRNA＆nbsp;in the periodontal tissue during orthodontic tooth movement. METHODS： Totaly 110 Sprague-Dawley rats were randomly divided into blank control group （n=10）, normal saline group （n=25）, 0.5 mg/kg nicotine group （n=25）, 0.75 mg/kg nicotine group （n=25）, 1 mg/kg nicotine group （n=25）. Rats in the normal saline groups were injected intraperitonealy with 0.1 mL normal saline, and those in the three nicotine groups were respectively injected with 0.5, 0.75, 1 mg/kg nicotine tartrate solution. Except the blank control group, the unilateral maxilary first molars of rats in the other four groups were exposed to 50 g force. At 1, 3, 5, 7, 14 days under force, the rats were sacrificed to take the maxilary tissues. Hematoxylin-eosin staining was used to observe the changes of periodontal tissues, immunohistochemical staining was employed to count positive cels, andin situ hybridization staining was adopted to detect the mean absorbance value of cyclooxygenase-2 in the periodontal tissues. RESULTTS AND CONCLUSION：The number of odontoclasts and the expression of cyclooxygenase-2 in the nicotine groups were higher than those in the non-nicotine groups. With the increasing dose of nicotine, the number of odontoclasts gradualy increased, and the difference was statisticaly significant （P 〈 0.05）. At 7 days under force, the number of odontoclasts reached the peak. With the increasing dose of nicotine, the positive expression intensity of cyclooxygenase-2 was also increased, and the difference was statisticaly significant （P 〈 0.05）. The expression of cyclooxygenase-2 reached peak at 5 days under force. These findings indicate that with the increasing dose of nicotine, the number of odontoclasts and the expression i

关 键 词：组织工程 尼古丁 尼古丁酒石酸溶液 正畸牙移动 免疫组织化学 原位杂交 环氧化酶2 mRNA 破牙骨质细胞 牙根吸收 牙周组织 

分 类 号：R318[医药卫生—生物医学工程]