4种药物干预治疗骨质疏松模型大鼠骨三点弯曲力学特性的对比分析  

Three-point bending mechanical properties of the bone in a rat model of osteoporosis after treatment with four kinds of drugs

作　　者：杨琦[1] 彭传刚[2] 王野[1] 王强[3] 

机构地区：[1]吉林大学中日联谊医院妇产科,吉林省长春市130000 [2]吉林大学第二医院骨科,吉林省长春市130026 [3]吉林大学第二医院妇产科,吉林省长春市130026

出　　处：《中国组织工程研究》2015年第46期7440-7444,共5页Chinese Journal of Tissue Engineering Research

摘　　要：背景:力学性能指标是评价药物治疗骨质疏松动物模型效果的重要方法,以3点弯曲力学性能指标评价多种药物治疗老龄雌性骨质疏松模型动物的效果鲜有报道。目的:以大鼠骨3点弯曲力学性能指标评价骨质疏松模型大鼠经丹杞颗粒、结合性雌激素、依普拉芬及αD_3干预的效果。方法:Wistar雌性大鼠48只分为6组,除正常组外,采用以去双侧卵巢的方法复制老龄骨质疏松大鼠模型。丹杞颗粒干预组每日给服丹杞颗粒0.9 g/kg,普拉芬干预组每日给服依普拉芬1 mg/kg,αD_3干预组每日给服αD_3 0.1 mg/kg,结合型雌激素干预组每日给服结合型雌激素0.3 mg/kg。以电子万能实验机对各组大鼠左、右侧胫骨进行3点弯曲力学性能测试。结果与结论:丹杞颗粒、依普拉芬、结合型雌激素干预组胫骨最大载荷、最大应力、最大弯矩、最大应力及弹性模量大于模型组(P<0.05);αD_3干预组最大载荷、最大应力、最大应变、弹性模量与模型组差异无显著性意义(P>0.05);丹杞颗粒干预组胫骨最大载荷、最大应力、最大应变与正常对照组比较差异无显著性意义(P>0.05)。丹杞颗粒干预组、结合型雌激素干预组、依普拉芬干预组弯曲力学性能恢复较好,以丹杞颗粒干预组效果最好,αD_3干预治疗组弯曲力学性能指标无明显恢复。结果说明以丹杞颗粒干预骨质疏松模型大鼠胫骨3点弯曲力学性能恢复效果最好。BACKGROUND：Mechanical property test is an important method for assessing the therapeutic effect of drug therapy in osteoporosis animal models. But there are rare reports on the three-point bending mechanical test for evaluating the therapeutic effects of a variety of drugs on a female rat model of senile osteoporosis. OBJECTIVE： To explore the interventional effects ofDanqi particles, premarin, ipriflavone and αD3 in a rat model of osteoporosis through the three-point bending mechanical test. METHODS：Forty-eight female Wistar rats were randomized into six groups, and animal models of senile osteoporosis were made in al rats except for those in the normal control group. Then, the rats were givenDanqi particles （0.9g/kg/d）, ipriflavone （1 mg/kg/d）, αD3 （0.1 mg/ka/d） and premarin （0.3 mg/kg/d） in theDanqi, ipriflavone, αD3 and premarin groups, respectively. An electronic universal testing machine was used to perform the three-point bending mechanical test on the bilateral tibiae of rats. RESULTS AND CONCLUSION： Compared with the model group, the maximum load, maximum stress, maximum bending moment, maximum stress, elastic modulus of the tibia were significantly higher in theDanqi, ipriflavone and premarin intervention groups （P〈 0.05）. There were also no significant differences in the maximum load, maximum stress and maximum strain between the Danqi and normal control groups （P〉 0.05）. These findings indicate thatDanqi, ipriflavone and premarin interventions have good achievements in the three-point bending mechanical test, and theDanqi particles have the best intervention effects. αD3 has no obvious effects on the three-point bending mechanical performance.

关 键 词：组织构建 骨组织工程 骨质疏松 动物模型 药物治疗 弯曲 力学特性 

分 类 号：R318[医药卫生—生物医学工程]