归巢因子基质细胞衍生因子1/CXCR4在角膜上的分布  

Corneal distribution of stromal cell-derived factor-1 and chemokine receptor-4 as homing factors

作　　者：张伟[1] 黄文志[1] 张玉平[2] 

机构地区：[1]广州市第一人民医院老年病科眼科,广东省广州市510180 [2]广州市第一人民医院血液病科,广东省广州市510180

出　　处：《中国组织工程研究》2015年第46期7466-7471,共6页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学青年科学基金项目(81300733);课题名称:角膜缘干细胞移植后内外源性干细胞归巢行为及调控机制的研究~~

摘　　要：背景:基质细胞衍生因子1/CXCR4在介导骨髓移植等组织器官的干细胞归巢中起着重要的作用。对于角膜干细胞移植的归巢和目前角膜上基质细胞衍生因子1/CXCR4的具体分布及含量较为少见。目的:荧光定量PCR检测大鼠角膜组织中基质细胞衍生因子1/CXCR4基因mR NA的表达水平。方法:取完整正常大鼠角膜组织,细分为角膜中央区,角膜旁中央及角膜缘区,用荧光定量PCR法(染料法)检测相应部位基质细胞衍生因子1/CXCR4基因mR NA的表达水平。结果与结论:角膜中央区基质细胞衍生因子1及CXCR4基因mR NA的表达水平分别为0.213±0.015,0.189±0.02;角膜旁中央区基质细胞衍生因子1及CXCR4基因mR NA的表达水平分别为0.529±0.077,0.285±0.015;角膜缘区基质细胞衍生因子1及CXCR4基因mR NA的表达水平分别为0.666±0.069,0.258±0.067。结果表明,在大鼠角膜组织中,角膜中央区,周边区及边缘区相应基质细胞衍生因子1及CXCR4的基因mR NA的表达水平均存在着显著性的差异,这种差异性对于角膜缘干细胞缺乏症干细胞移植治疗中细胞的归巢及移行起着重要的作用。BACKGROUND：Stromal cel-derived factor-1 （SDF-1） and chemokine receptor-4 （CXCR4） play an important role in stem cel homing during bone marrow, organ and tissue transplantation. But there are few reports on limbal stem cel transplantation and homing as wel as the distribution and content of SDF-1/CXCR4 on the cornea. OBJECTIVE：To detect the expression levels of SDF-1/CXCR4 mRNA in rat corneal tissue by fluorescence quantitative PCR. METHODS： The normal rat cornea was taken completely, and subdivided into central cornea, peripheral corneal and limbal area. Fluorescence quantitative PCR method was employed to detect SDF-1/CXCR4 mRNA levels at corresponding positions. RESULTS AND CONCLUSION： The expression levels of SDF-1 and CXCR4 mRNA were 0.213±0.015 and 0.189±0.02 in the central cornea, 0.529±0.077 and 0.285±0.015 in the peripheral cornea, 0.666±0.069 and 0.258±0.067 in the limbal area, respectively. These data show that there are significant differences in the expression levels of SDF-1 and CXCR4 mRNA in the central cornea, peripheral corneal and limbal area, and these differences exert an important role in cel homing and migration during limbal stem cel transplantation for treatment of limbal stem cel deficiency.

关 键 词：组织构建 组织工程 角膜 大鼠 归巢 SDF-1/CXCR4 荧光定量PCR 国家自然科学基金 

分 类 号：R318[医药卫生—生物医学工程]