人血管内皮细胞生长因子121基因修饰材料修复桡骨火器性骨缺损  

Human vascular endothelial growth factor 121 gene-modified materials repair firearm-induced radial defects

作　　者：王建忠[1] 李兵仓[2] 任宝[1] 高飞[1] 李聪杰[1] 刘伟[1] 王晓冬[1] 雷鸣[1] 高文山[1] 

机构地区：[1]河北大学附属医院,河北省保定市071000 [2]解放军第三军医大学大坪医院野战外科研究所第6研究室创伤烧伤复合伤国家重点实验室,重庆市400042

出　　处：《中国组织工程研究》2015年第47期7561-7566,共6页Chinese Journal of Tissue Engineering Research

基　　金：全军医学科学技术研究"十五"指令性课题(01L060);河北省科学技术研究与发展计划项目(072761417)~~

摘　　要：背景:骨缺损是导致火器性股损伤骨不连的首要原因,火器性损伤相对比较特殊。自体骨移植和异体骨移植均存在较大弊端,难以满足基层医院需求,采用具有良好血管能力、骨能力的组织工程化骨修复火器性骨缺损将是一种理想的、可行的修复方法。目的:探讨人血管内皮细胞生长因子121基因修饰材料在兔桡骨火器性骨缺损修复中的应用。方法:将128只家兔随机分为手术致伤组和火器伤组,每组64只。火器伤组采用56式滑膛枪发射质量为0.25 g的钢珠,建立火器伤桡骨损伤模型;手术致伤组仅用手术方法造成兔桡骨1.2 cm损伤模型。采用人血管内皮细胞生长因子121基因修饰材料进行修复处理,取家兔相关组织细胞,获得骨髓基质干细胞进行培养,制备多孔支架材料,并将获得的材料分别植入手术致伤组和火器伤组家兔桡骨骨缺损部位,分析人血管内皮细胞生长因子121基因修饰材料在兔桡骨火器性骨缺损修复中的应用。结果与结论:与手术致伤组相比,修复8,12,16周后,火器伤组家兔骨缺损部位灰度比值、健侧与修复侧桡骨的抗压缩力学比值降低(P<0.05),新生骨面积增多(P<0.05);修复2,4周后,骨缺损修复处局部血流量显著升高(P<0.05)。结果说明,与单纯手术致桡骨损伤相比,烧伤致桡骨损伤修复过程中由于局部出现了缺血缺氧,采用人血管内皮细胞生长因子121基因治疗效果更理想。人血管内皮细胞生长因子121能够对骨髓基质细胞进行修复,将其运用于火器烧伤中能够提高其合成及分泌成血管因子,提高其局部血流量、降低抗压缩力学比值,且新生骨面积较大。BACKGROUND：Bone defects are the leading cause of nonunion after firearm injury. Firearm injury is relatively special. Autograft and alograft al have big drawbacks, which cannot meet the requirements of basic-level hospitals. Using tissue-engineered bone with good blood vessels and osteogenic capability in repair of firearm＆nbsp;bone defect wil be an ideal and feasible restoration method. OBJECTIVE： To explore the application of human vascular endothelial growth factor 121 gene-modified materials in the repair of firearm-induced radial injury in rabbits. METHODS： A total of 128 rabbits were randomly divided into surgical injury group and firearm injury group （n=64 per group）. In the firearm injury group, 0.25 g steel bal was launched using 56-style musket to establish a firearm radial injury model; in the surgical injury group, surgical methods were used to produce a 1.2 cm radial injury model. Human vascular endothelial growth factor 121 gene-modified materials were used. The related histocytes from rabbits were harvested to obtain bone marrow stromal cels for culture. A porous scaffold material was prepared. The obtained materials were respectively implanted into radial defect sites in the surgical injury and firearm injury groups. The application of human vascular endothelial growth factor 121 gene-modified materials in rabbit radial defect repair was analyzed. RESULTS AND CONCLUSION： Compared with the surgical injury group, at 8, 12 and 16 weeks after repair, the gray level ratio of bone defect site and the anti-compression mechanical ratio at the healthy and repairing sides of the radius in the firearm injury group were decreased （P 〈 0.05）, and the new bone area increased （P 〈 0.05）. At 2 and 4 weeks after repair, the local blood flow at the repair area was significantly increased （P 〈 0.05）. These results suggest that compared with the surgical injury group, the curative effect of human vascular endothelial growth factor 121 gene is more ideal in the firearm injury group beca

关 键 词：生物材料 骨生物材料 人血管内皮细胞生长因子121基因 修饰材料 兔桡骨火器性骨缺损 骨髓基质干细胞 局部血流量 灰度比值 抗压缩力学 血管因子 聚乳酸-聚羟基乙酸共聚物 新生骨面积 

分 类 号：R318[医药卫生—生物医学工程]