矿化明胶静电纺丝诱导牙周组织成骨的有效性  

Effectiveness of mineralized gelatin electrostatic spinning on inducing periodontal tissue osteogenesis

作　　者：郭瑞征[1] 王亚丽[1] 

机构地区：[1]中国人民解放军第二五四医院口腔科,天津市300142

出　　处：《中国组织工程研究》2015年第47期7583-7588,共6页Chinese Journal of Tissue Engineering Research

摘　　要：背景:当前国内外关于矿化明胶电纺丝纤维对牙周组织成骨诱导有效性的文献较少。目的:观察矿化明胶静电纺丝对牙周膜成纤维细胞增殖及向成骨方面分化的影响。方法:将人牙周膜成纤维细胞分别与未矿化明胶静电纺丝、纳米羟基磷灰石矿化1 d的明胶静电纺丝、纳米羟基磷灰石矿化5 d的明胶静电纺丝复合培养,采用MTT法检测培养1,4,7,10,13 d的细胞增殖;应用生化仪检测培养1,7,14 d的细胞碱性磷酸酶活性。结果与结论:培养10 d时,矿化明胶静电纺丝膜组表面的牙周膜细胞分布均匀,生长良好,铺展成片状并分泌大量细胞外基质,细胞与材料结合紧密,且以矿化5 d组效果更明显,细胞生长密度和状态优于未矿化明胶静电纺丝膜组。不同时间点的细胞增殖活性与碱性磷酸酶活性:矿化5 d明胶静电纺丝膜组>矿化1 d明胶静电纺丝膜组>未矿化明胶静电纺丝膜组(P均<0.05)。表明经纳米羟基磷灰石矿化的明胶静电纺丝,可促进牙周膜成纤维细胞增殖及向成骨方向分化,且矿化时间越长效果越明显。BACKGROUND：Currently, little is reported regarding the effectiveness of mineralized gelatin electrostatic spinning fibers on periodontal tissue osteogenic induction. OBJECTIVE： To investigate the effect of mineralized gelatin electrostatic spinning on the proliferation and osteogenic differentiation of periodontal ligament fibroblasts. METHODS： Human periodontal ligament fibroblasts were respectively compositely cultured with non-mineralized gelatin electrostatic spinning, gelatin electrostatic spinning after 1 day of nano-hydroxyapatite mineralization and gelatin electrostatic spinning after 5 days of nano-hydroxyapatite mineralization. Cel proliferation was determined at 1, 4, 7, 10 and 13 days of culture using MTT assay. Alkaline phosphatase activity of cels was determined at 1, 7, 14 days of culture using biochemical analyzer. RESULTS AND CONCLUSION：At the 10th day of culture, the periodontal ligament cels on the surface of membrane distributed uniformly, grew wel, spread out as sheets and secreted a large amount of extracelular matrix, linked closely with the materialsl; moreover, the effect was more obvious at 5 days of mineralization. Cel growth density and status were better than those in the non-mineralized gelatin electrostatic spinning membrane group. Cel proliferation and alkaline phosphatase activities at different time points： 5-day mineralized gelatin electrostatic spinning membrane group 〉 1-day mineralized gelatin electrostatic spinning membrane group 〉 non-mineralized gelatin electrostatic spinning fibers group （alP 〈 0.05）. These results demonstrate that the gelatin electrostatic spinning after nano-hydroxyapatite mineralization may promote the proliferation and osteogenic differentiation of periodontal ligament fibroblasts in a time-dependent manner.

关 键 词：生物材料 口腔生物材料 矿化明胶静电纺丝 牙周组织 牙周膜成纤维细胞 增殖 成骨诱导 

分 类 号：R318[医药卫生—生物医学工程]