远隔缺血后处理对脑缺血模型大鼠再灌注损伤炎性反应相关信号的影响  

Effects of remote ischemic postconditioning on inflammation related factors of cerebral ischemia/reperfusion injury

作　　者：王宁[1] 李晓欧[1] 巴晓红[2] 

机构地区：[1]辽宁医学院,辽宁省锦州市121001 [2]辽宁医学院附属第一医院神经内科,辽宁省锦州市121001

出　　处：《中国组织工程研究》2015年第49期7902-7907,共6页Chinese Journal of Tissue Engineering Research

基　　金：辽宁省科技厅基金资助项目(2014022006)~~

摘　　要：背景:虽然目前已有一些研究表明远隔缺血后处理可以发挥神经保护作用,但是具体的机制尚不明了。目的:探讨远隔缺血后处理对大鼠局灶性脑缺血再灌注损伤的保护作用。方法:应用线栓法制备大鼠大脑中动脉闭塞局灶性脑缺血再灌注模型,并进行远隔缺血后处理,同时设假手术组和缺血再灌注组作对照。于缺血再灌注24 h后进行神经功能评分,检测梗死体积及脑含水量;RT-PCR检测缺血周围区脑组织内白细胞介素1β、白细胞介素6、肿瘤坏死因子α和单核细胞趋化蛋白1 mRNA表达情况;Western blot检测Bcl-2和Bax蛋白表达情况。结果与结论:与缺血再灌注组相比,远隔缺血后处理组神经功能评分有所降低,但差异无显著性意义,梗死范围和脑组织含水量显著降低(P<0.05)。远隔缺血后处理组与缺血再灌注组相比,大鼠缺血周围区脑组织白细胞介素1β、白细胞介素6、肿瘤坏死因子α、单核细胞趋化蛋白1 mRNA和Bax蛋白表达降低(P<0.05),Bcl-2蛋白表达升高(P<0.05)。结果证实,远隔缺血后处理可以减轻大鼠局灶性脑缺血再灌注产生的损伤,其机制可能与减轻炎性反应有关。BACKGROUND： Although some studies have suggested that remote ischemic postconditioning plays a neuroprotective role, but the specific mechanism is still unknown.OBJECTIVE： To investigate the protective effects of remote ischemic postconditioning on focal cerebral ischemia/reperfusion injury in rats. METHODS： Focal cerebral ischemia/reperfusion injury rat models were established by occlusion of the middle cerebral artery using suture method and were subjected to remote ischemic postconditioning. Meanwhile, sham and ischemia/reperfusion groups were established as comparison. After 24 hours of ischemia/reperfusion, neurological function was graded to determine cerebral infarction volume and brain water content. The mRNA expression of interleukin 1β, interleukin-6, tumor necrosis factor α and monocyte chemoattractant protein 1 in brain tissue around ischemic area was detected by RT-PCR. The protein expression of Bcl-2 and Bax was detected by western blot analysis. RESULTS AND CONCLUSION： Compared with ischemia/reperfusion group, the neurological function scores in the remote ischemic postconditioning group were slightly, but not significantly, decreased. Infarct volume and brain water content in remote ischemic postconditioning group were significantly decreased compared with those in ischemia/reperfusion group（P 〈0.05）. The mRNA expression of interleukin 1β, interleukin-6, tumor necrosis factor α and monocyte chemoattractant protein 1 and the protein expression of Bax in brain tissue aroundischemic area in ischemic postconditioning group were significantly decreased compared with those in ischemia/reperfusion group（P 〈0.05）. Bcl-2 protein expression significantly increased（P 〈0.05）. These results demonstrate that remote ischemic postconditioning can relieve focal cerebral ischemia/reperfusion injury in rats, the mechanism of which may be associated with reduction of inflammatory response.

关 键 词：组织工程 模型 动物 脑缺血 细胞凋亡 实验动物模型 脑及脊髓损伤动物模型 缺血再灌注 远隔缺血后处理 炎性 大鼠 损伤 神经功能 梗死面积 含水量 凋亡 

分 类 号：R318[医药卫生—生物医学工程]