不同脊髓损伤模型大鼠行为学及形态学的变化  

Behavioral and morphological changes of different spinal cord injury rat models

作　　者：宋伟[1,2] 段红梅[3] 饶家声[3] 赵璨[3] 杨朝阳[4] 

机构地区：[1]首都医科大学康复医学院,北京市100068 [2]中国康复研究中心康复工程研究所,北京市100068 [3]北京航空航天大学生物与医学工程学院,北京市100191 [4]首都医科大学北京神经科学研究所,北京市100054

出　　处：《中国组织工程研究》2015年第49期7920-7925,共6页Chinese Journal of Tissue Engineering Research

基　　金：国家科技支撑计划项目(2012BAI17B00)~~

摘　　要：背景:在脊髓全断模型中有切割伤模型、切除伤模型,为了更好地选择脊髓损伤修复研究的全断动物模型,有必要对这两种模型进行比较及探讨。目的:分析不同脊髓损伤模型的行为学及病理变化,选择适用于再生研究及治疗的脊髓损伤动物模型。方法:将160只SD大鼠随机均分为2组,分别制作切割型脊髓损伤模型与切除型脊髓损伤模型,术后1,2,4,8,10,20,30,50周内进行后肢行为学BBB评分及组织化学、免疫组织化学染色,观察损伤区残余纤维情况;术后4,10,26,52周分别在大鼠脑皮质运动区与颈髓注射BDA-FITC示踪剂,观察损伤区残余纤维的连续性。结果与结论:切割脊髓损伤模型组大鼠术后2-50周双后肢的BBB得分明显高于切除脊髓损伤模型组(P<0.05);切割脊髓损伤模型组在损伤区残存大量组织和神经丝、胶质纤维酸性蛋白纤维,而切除脊髓损伤模型几乎没有残存纤维;不论是切割脊髓损伤模型还是切除脊髓损伤模型,皮质脊髓束均不能通过损伤区,终止于损伤区的头端;在切割脊髓损伤模型中,脊髓固有束重新进入尾侧端的宿主脊髓组织中,而在切除脊髓损伤模型中脊髓固有束终止于损伤区的头端。表明切除脊髓损伤模型更适用于脊髓损伤后再生效果及治疗手段或药物的筛选与评价。BACKGROUND： Complete spinal cord transection models include cut-type injury and excision-type injury models. It is necessary to compare and discuss these two models, in order to select more appropriate animal models for studying spinal cord injury and repair.OBJECTIVE： To analyze the behavioral and pathological changes of different spinal cord injury models and to select the animal models of spinal cord injury which are more suitable for studying regeneration and treatment of injured spinal cord. METHODS： One hundred and sixty Sprague-Dawley rats were randomly divided into two groups to establish cut-type and excision-type spinal cord injury models. At 1, 2, 4, 8, 10, 20, 30 and 50 weeks after operation, rat hindlimb locomotor skills were evaluated using BBB scores; histochemical staining and immunohistochemical staining were conducted to observe the residual fibers in injured area; at 4, 10, 26 and 52 weeks after operation, BDA-FITC tracer agent was respectively injected into the motor area of the cerebral cortex and cervical cord of rats, to further observe the continuity of residual fibers in injured area. RESULTS AND CONCLUSION： The BBB scores of rat hind limbs in cut-type spinal cord injury model group from 2 to 50 weeks after operation were significantly higher than those in excision-type spinal cord injury model group（P 0.05）. In cut-type spinal cord injury model group, there were a large amount of residual tissues, neurofilaments and glial fibrillary acidic protein-positive fibers in injured area, but there were almost no residualfibers in excision-type spinal cord injury model group. The corticospinal tract could pass through the injured area in neither cut-type spinal cord injury models nor excision-type spinal cord injury models and terminated at the cranial end of the injured area. In cut-type spinal cord injury models, the spinal cord propriospinal tract re-entered into the host spinal cord tissue at the caudal end, whereas the spinal cord propriospinal tract in excision-type spinal cord

关 键 词：脊髓损伤 模型 动物 神经元示踪剂 组织工程 实验动物模型 脑及脊髓损伤动物模型 病理变化 功能恢复 神经示踪 

分 类 号：R318[医药卫生—生物医学工程]