光化学栓塞法建立缺血性脑卒中动物模型  

Establishing an animal model of ischemic stroke with photothrombosis method

作　　者：马浚宁 高俊玮[1,2] 侯博儒[1] 任海军[1,2] 刘吉星[1] 陈四化[1] 严贵忠 

机构地区：[1]兰州大学第二临床医院神经外科,甘肃省兰州市730000 [2]兰州大学生命科学院,甘肃省兰州市730000

出　　处：《中国组织工程研究》2015年第49期7951-7957,共7页Chinese Journal of Tissue Engineering Research

基　　金：甘肃省自然科学基金(145RJZA146;1208RJZA184)~~

摘　　要：背景:实验性缺血性脑卒中动物模型是研究缺血性脑卒中病理机制以及研究开发新治疗方案必不可少的工具,光化学栓塞法制作缺血性脑卒中动物模型操作简便,稳定性好,重复性高,可有针对性的控制梗死灶的位置以及大小,是研究缺血性脑卒中病理机制的良好选择。目的:建立光化学栓塞法缺血性脑卒中动物模型,对比不同性别小鼠缺血性脑卒中后梗死体积以及行为学表现的异同。方法:62只昆明小鼠依据检测内容分为病理学检测6只、梗死体积测试16只和行为学测试40只。其中病理学检测小鼠分为假手术组和模型组,各3只;梗死体积测试小鼠分为雌性模型组和雄性模型组,各8只;行为学测试小鼠分为雌性模型组、雌性假手术组、雄性模型组和雄性假手术组,各10只。模型组(含雌性模型组和雄性模型组)小鼠采用光化学栓塞法制备局部缺血性卒中模型,假手术组(含雌性假手术组和雄性假手术组)小鼠不注射孟加拉玫瑰红染料。结果与结论:尼氏染色显示模型组小鼠梗死中心出现神经元变性坏死,空泡样病理改变,Fluoro-Jade C染色可见模型组小鼠梗死中心中出现变性神经元,且雌性小鼠脑梗死体积显著小于雄性小鼠,模型组小鼠行为运动功能受损程度显著大于假手术组小鼠,其中雌性小鼠运动功能受损程度小于雄性小鼠。表明光化学栓塞法可以成功建立缺血性脑卒中动物模型,且相同条件下缺血性脑卒中对于雄性造成的神经功能损伤更为严重。BACKGROUND： Animal model of experimental cerebral ischemia stroke is an indispensable tool in the research of stroke pathology and the development of novel treatment. The animal model of cerebral ischemia stroke established with the photothrombosis method has simple operation, good stability and high repeatability, it can control the position and size of infarction loci, and therefore it is the preferred choice for studying the pathological mechanism of cerebral ischemic stroke.OBJECTIVE： To establish animal models of cerebral ischemic stroke with the photothrombosis model, and compare the infarct volume and behaviors of difference genders of mice after ischemic stroke. METHODS： Totally 62 Kunming mice were included in this study, 6 of them were used for the pathology detection, 16 of them for the measurement of infarction volume, and 40 for behavioral test. In the pathology detection, 6 mice were equally divided into sham group and model group; in the measurement of infarction volume, 16 mice were equally divided into female model group and male model group; in the behavioral test, 40 mice were equally divided into female model group, female sham group, male model group and male sham group. In the model groups（female model group and male model group）, focal cerebral ischemic stroke model was establishedwith the photothrombosis method; in the sham groups（female sham group and male sham group）, mice were not injected with rose Bengal dye. RESULTS AND CONCLUSION： Nissl staining found neuronal degeneration, necrosis, and vacuolar pathology at the center of infarction loci in the model groups. Fluoro-Jade C staining shown neuronal degeneration at the center of infarction loci in the model groups. Infarct volume in female group was significantly smaller than that in male group. The motor function impairment in the model group was significantly greater than that in sham group, and female group showed less impairment of motor function than male group. These results suggest that the animal model of ischem

关 键 词：卒中 光化学 性别因素 行为 组织工程 实验动物模型 脑及脊髓损伤动物模型 光化学法 缺血性脑卒中 动物模型 尼氏染色 FLUORO-JADE C染色 行为学 疲劳转棒 前肢抓力 甘肃省自然科学基金 

分 类 号：R318[医药卫生—生物医学工程]