实验性牙移动模型大鼠构建:牙齿移动后空口咀嚼时间变化规律  

Construction of rat models of experimental tooth movement:time variation of chewing-like jaw movements after tooth movement

作　　者：栗洪师[1] 陈新[1] 冯岩[1] 刘洪臣[2] 

机构地区：[1]解放军空军总医院口腔科,北京市100142 [2]解放军总医院口腔研究所,北京市100138

出　　处：《中国组织工程研究》2015年第49期7988-7993,共6页Chinese Journal of Tissue Engineering Research

基　　金：首都临床特色应用研究与成果推广项目(Z151100004015210);空军总医院院内课题(KZ2015013)~~

摘　　要：背景:错颌畸形正畸交互牵引移动牙齿产生有规律自发性疼痛,但是疼痛的中枢神经传导通路以及疼痛的基本机制并不清楚。目的:建立实验性牙移动模型大鼠,观察大鼠牙移动后单位时间空口咀嚼行为学变化规律。方法:将大鼠随机分为空白组,阴性对照组和模型组,模型组使用改良Colin.K.法,用镍钛丝正畸矫治力交互牵引大鼠上颌前后牙建立大鼠牙移动模型;空白组无交互牵引装置;阴性对照组不加矫治力;分别检测在牙移动后4,12 h,移动后1,3,5,7 d大鼠空口咀嚼行为学改变;牙移动后1 d,牙齿移动分别加力30,60,90 g,观察大鼠空口咀嚼相关行为学变化。结果与结论:与阴性对照组和空白组比较,模型组大鼠牙移动后4 h开始,空口咀嚼时间总和开始增加,牙移动后12 h空口咀嚼时间明显增加(P<0.05),牙移动后1 d达到峰值(P<0.01),随后缓慢下降至7 d。牙齿移动后1 d,模型组大鼠施加正畸矫治力30,60,90 g的空口咀嚼时间均差异有显著性意义(P<0.05)。结果证实,牙齿移动后空口咀嚼时间变化规律和临床正畸牙移动疼痛规律一致,能够作为大鼠牙移动后口颌面部疼痛相关行为学反应之一。BACKGROUND： Tooth movement induced by interactive traction during orthodontic treatment causes regular spontaneous pain, however, the central nervous system conduction pathway and the basic mechanisms of pain are not clear.OBJECTIVE： To establish rat models of experimental tooth movement and observe the behavioral variation of rat chewing-like jaw movements in unit time after tooth movement. METHODS： Rats were randomly divided into blank, negative control and model groups. According to modified Colin. K. method, rat models of experimental tooth movement were established in the model group through interactive traction of the rat maxillary anterior and posterior teeth by orthodontic nickel-titanium wires. Rats in the blank group were not subjected to interactive traction treatment. Rats in the negative control group were not imposed corrective force. The behavioral variation of chewing-like jaw movements of rats was detected respectively at 4, 12 hours and at 1, 3, 5, and 7 days after tooth movement. At 1 day after tooth movement, relative variation of chewing-like jaw movements of rats was observed after imposing orthodontic force of 30, 60 and 90 g on movable tooth. RESULTS AND CONCLUSION： Beginning from 4 hours after tooth movement, the total time of chewing-like jaw movements began to increase, and the time of chewing-like jaw movements was significantly increased at 12hours after tooth movement, reached the peak at 1 day after tooth movement, and then was slowly declined until 7 days in the model group compared with that in the negative control and blank groups. At 1 day after tooth movement, there was significant difference in the time taken for chewing-like jaw movements in the model group among orthodontic force of 30, 60 and 90 g（P 〈0.05）. These results confirm that the time variation of chewing-like jaw movements after tooth movement is consistent with the rule of pain induced by tooth movement during clinical orthodontic treatment, which can be used as one of the related behavioral response

关 键 词：组织工程 牙齿 疼痛 模型 动物 实验动物模型 口腔损伤及修复动物模型 实验性牙齿移动 正畸矫治力 错颌畸形 交互牵引 自发疼痛 空口咀嚼 直接梳理面部 变化规律 

分 类 号：R318[医药卫生—生物医学工程]