角膜炎模型大鼠白细胞介素10及环氧酶2的表达  

Expression of interleukin-10 and cyclooxygenase 2 in keratitis rat models

作　　者：陆霞[1] 潘旭斌[1] 谢玉涛[2] 季秋娣 

机构地区：[1]无锡市第四人民医院眼科,江苏省无锡市214062 [2]无锡市第四人民医院手术室,江苏省无锡市214062 [3]无锡市第四人民医院皮肤科,江苏省无锡市214062

出　　处：《中国组织工程研究》2015年第49期8015-8020,共6页Chinese Journal of Tissue Engineering Research

摘　　要：背景:研究白细胞介素10、环氧酶2与模型大鼠角膜炎发生发展的关系,对角膜炎的治疗有重大意义。目的:检测白细胞介素10及环氧酶2在大鼠模型角膜炎中的表达情况,分析白细胞介素10、环氧酶2与模型大鼠角膜炎发生发展的关系。方法:随机选取26只健康大鼠,不限雌雄,大鼠左眼选为实验组,采用角膜表面镜片术法建立角膜炎感染模型;右眼作为正常对照组。建模后第1,3,7及14天摘取角膜组织,观察大鼠左右眼角膜组织病理学变化,并采用免疫组织化学及反转录聚合酶链式反应(RT-PCR)法,检测白细胞介素10及环氧酶2在不同程度角膜炎的角膜组织中的表达情况。结果与结论:1组织病理学观察显示角膜炎病灶有严重组织坏死及中性粒细胞浸润,PAS染色结果显示菌丝主要存在于角膜基质层。2白细胞介素10在对照组大鼠角膜中基本不表达,感染角膜炎后,白细胞介素10的表达先上升后下降,各时间点有显著性差异(P<0.01)。3环氧酶2在对照组大鼠角膜中基本不表达,感染角膜炎后,环氧酶2的表达逐渐升高,第14天降低,各时间点有显著性差异(P<0.01)。PAS染色及病变组织培养实验证明建立大鼠模型角膜炎成功。结果显示白细胞介素10在角膜炎病变后期有表达,推测其可能参与角膜炎后期的组织损伤修复;环氧酶2的表达位于角膜炎组织,是角膜炎病变的敏感炎症因子。BACKGROUND： Investigating the relationship between interleukin-10, cyclooxygenase-2 expression levels and the occurrence and development of keratitis is of great significance for the treatment of keratitis. OBJECTIVE： To detect the expression of interleukin-10 and cyclooxygenase 2 in keratitis rat models and analyze the relationship between interleukin-10, cyclooxygenase-2 expression and the occurrence and development of keratitis in rats.METHODS： Twenty-six healthy rats of either gender were randomly selected to establish keratitis rat models. The left eyes of rats were chosen as experimental group to establish keratitis models using epikeratophakia. The right eyes of rats were set as control group. At the 1^th, 3^rd, 7^th and 14^th days after modeling, the pathological changes of rat corneal tissue were observed in both eyes. The expression of interleukin-10 and cyclooxygenase 2 in the corneal tissue with different degrees of keratitis was determined using immunohistochemistry and reverse transcription-polymerase chain reaction（RT-PCR）. RESULTS AND CONCLUSION： Histopathological observation showed that there were severe tissue necrosis and neutrophil infiltration in keratitis foci. PAS staining showed that the mycelium was mainly present in the corneal stroma. In the control group, the expression of interleukin-10 was hardly detected in rat cornea. After keratitis infection, the expression of interleukin 10 increased at first and then decreased, and there was significant difference between different time points（P〈 0.01）. In the control group, the expression of cyclooxygenase-2 was hardly detected in rat cornea. After keratitis infection, the expression of cyclooxygenase 2 was increased gradually and then decreased at the 14^th day. There was significant difference between different time points（P 〈0.01）. PAS staining and pathologic tissue culture experiment suggest that rat models of keratitis were successfully established. These results show that the expression of interleukin-10 appeared

关 键 词：角膜炎 白细胞介素10 环氧酶2 组织工程 实验动物模型 基因病毒载体及相关因子动物模型 

分 类 号：R318[医药卫生—生物医学工程]