垂直层叠结构的LED诱导荧光检测系统  

Vertical Multi-Layer LED-Induced Fluorescence Detection System

作　　者：杨晓博[1,2] 闫卫平[2] 

机构地区：[1]许昌学院电气工程学院,河南许昌461000 [2]大连理工大学电子科学与技术学院,辽宁大连116024

出　　处：《仪表技术与传感器》2015年第3期66-68,81,共4页Instrument Technique and Sensor

基　　金：河南省科技厅基础与前沿技术研究计划项目(132300410091);河南省教育厅科学技术研究重点项目(13B510240)

摘　　要：为进一步减小光源的体积及简化光路系统结构,消除毛细管电泳芯片激光诱导荧光检测系统中激发光源的反射光和杂散光的干扰,降低系统的检测限,设计并建立了基于偏振隔离结构的垂直层叠式发光二极管诱导荧光检测系统。整个系统由发光二极管光源、两片线性偏振片、多通道毛细管电泳芯片、针孔、电荷耦合器件、高压电源、数据采集卡及数据处理单元等组成。在系统条件最优化的情况下,对罗丹明B样品溶液进行了毛细管电泳分离实验。A cross-polarization scheme and vertical multi-layer structure was presented to filter out the excitation light from the emission spectrum of fluorescent dyes using green light emitting diodes as a light source and a linear charge coupled device as an intensity detector in order to decrease detection limit of the system. The detection system was composed of light emitting diode light source,two polarizers,capillary array electrophoresis microchip,pinhole,charge coupled device,high-voltage power supply,data acquisition card and data processing unit. Under optimal conditions,capillary electrophoresis separation test of Rhodamine B dye sample was conducted.

关 键 词：垂直层叠结构 毛细管电泳芯片 荧光检测 偏振片 

分 类 号：TN29[电子电信—物理电子学]