黄芩醇提物干预D-半乳糖致衰老大鼠的尿液代谢组学研究  

Urinary metabolomics study of the effects of Scutellaria baicalensis Georgi ethanol extract on D-galactose-induced rats

作　　者：常艳芬[1,2] 宫文霞[1,2] 郑艳红[1,2] 李建伟[1,3] 周玉枝[1] 秦雪梅[1] 杜冠华[1,4] 

机构地区：[1]山西大学中医药现代研究中心,北京100050 [2]山西大学化学化工学院,山西太原030006北京100050 [3]山西振东制药股份有限公司,山西长治047100 [4]中国医学科学院药物研究所,北京100050

出　　处：《药学学报》2016年第1期86-92,共7页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目(30901960);山西省科技基础条件平台建设项目(2014091022);山西省科技攻关项目(20140313008-14);山西省重点技术创新项目[晋财建-(2013)99号]

摘　　要：研究黄芩对D-半乳糖致衰老模型大鼠的影响,初步探讨黄芩的抗衰老作用机制。将SD大鼠随机分为5组,即空白组、模型组、黄芩低、中、高剂量(分别为50、100和200 mg·kg^(-1))组,采用皮下注射D-半乳糖(100mg·kg^(-1))法,建立亚急性衰老大鼠模型,并分别评价衰老大鼠的空间学习记忆能力(Morris water maze)和自主活动(open-field test)。采用代谢组学技术对模型大鼠尿液进行NMR数据采集并结合多元统计分析,探讨抗衰老机制。结果表明,黄芩3个剂量均能改善衰老大鼠的行为能力。多元统计分析结果显示,黄芩低中高剂量组能使衰老大鼠尿液中柠檬酸、丙酮酸、乳酸、泛酸、三甲胺和β-羟基丁酸等6个标志物发生不同程度的回调,主要涉及能量代谢、糖代谢和肠菌代谢等代谢途径的调节,表明黄芩发挥抗衰老作用与这些途径相关。The purpose of this study is to evaluate the anti-aging effects and reveal the underlying mechanism of Scutellaria baicalensis Georgi ethanol extract（SBG） in D-galactose-induced rats. Fifty rats were randomly divided into five groups： vehicle control group, D-galactose group, and D-galactose combined with 50, 100, 200 mg·kg^（-1） SBG. A rat aging model was induced by injecting subcutaneously D-galactose（100 mg·kg^（-1）） for ten weeks. At the tenth week, the locomotor activity（in open-field test） and the learning and memory abilities（in Morris water maze test） were examined respectively. The urine was collected using metabolic cages and analyzed by high-resolution ~1H NMR spectroscopy combined with multivariate statistical analyses. The SBG at doses of 50, 100 and 200 mg·kg^（-1） treatments groups could significantly ameliorate aging process in rats＇ cognitive performance. The 50, 100, 200 mg·kg^（-1） SBG regulated citrate, pyruvate, lactate, trimethylamine（TMA）, pantothenate, β-hydroxybutyrate in urine favorably toward the control group. These biochemical changes are related to the disturbance in energy metabolism, glycometabolism and microbiome metabolism, which is helpful to further understanding the D-galactose induced aging rats and the therapeutic mechanism of SBG.

关 键 词：黄芩 抗衰老 行为学研究 代谢组学 

分 类 号：R917[医药卫生—药物分析学]