茎瘤芥(榨菜)子叶及带下胚轴茎段离体培养研究  

The study of cotyledon and hypocotyl with stem on Brassica juncea var.tumida Tsen et Lee in vitro culture

作　　者：朱学栋 何晓蓉 刘华强 杨霞 朱菲菲 周春江 

机构地区：[1]重庆市渝东南农业科学院中心实验室,重庆涪陵408000

出　　处：《安徽大学学报（自然科学版）》2016年第1期86-90,共5页Journal of Anhui University(Natural Science Edition)

基　　金：重庆市涪陵区科委项目(FLKJ;2014ABB2066)

摘　　要：以茎瘤芥永安小叶品种无菌苗子叶和带下胚轴茎段为材料,初步探索愈伤组织诱导、分化及再生植株生根培养.结果表明,子叶与叶柄的切口处极易产生愈伤组织,在MS+1.0mg·L-16-BA+0.2mg·L^(-1)NAA培养基中,愈伤组织分化率达到64%,其不定芽量最大;1/2MS+0.6mg·L^(-1)IBA培养基对不定根形成最为有利,生根率可达到96%.Cotyledon and hypocotyl stem section under as materials of Brassica juncea var.tumida Tsen et Lee named Yongan Xiaoye,are used to explore callus induction,differentiation and regeneration plant rooting culture preliminarily.The results showed that the slit of cotyledon and petiole produce callus.Cultured in MS ＋ 1.0 mg·L-1 6-BA ＋0.2mg·L-1 NAA medium,the callus differentiation rate reached to 64%.In 1/2 MS ＋0.6mg·L-1 IBA medium for adventitious root formation is most favorable,the rooting rate reached to 96%.

关 键 词：子叶 愈伤组织 分化 生根 

分 类 号：O156[理学—数学]