化学发光酶免疫分析法及ELISA检测抗-HCV的结果比较  

Comparison analysis of effects of chemiluminescent enzyme immunoassay and ELISA for detection of hepatitis C antibodies

作　　者：张利[1] 于冬男[2] 

机构地区：[1]内蒙古自治区赤峰市赤峰学院附属医院检验科,024005 [2]广东省人民医院,广州510080

出　　处：《检验医学与临床》2016年第1期18-20,共3页Laboratory Medicine and Clinic

基　　金：广东省医学科研基金立项课题(A2014054)

摘　　要：目的对比化学发光酶免疫分析法及酶联免疫吸附试验(ELISA)用于丙型肝炎抗体检测的效果。方法选取丙型肝炎患者140例,抽取血液标本后分别进行丙型肝炎病毒(HCV)相关抗体的化学发光酶免疫分析法及ELISA法检测。结果化学发光酶免疫分析法检出抗-HCV阳性的检出率为98.6%,高于ELISA法的检出率(94.3%),差异有统计学意义(P<0.05)。化学发光酶免疫分析法对于抗AMA-M2抗体、抗3E抗体、抗SP100抗体、抗PML抗体、抗GP210抗体的检测阳性率分别为80.0%、73.6%、37.9%、55.7%和47.9%,而ELISA法检测结果分别为12.9%、12.9%、13.6%、10.7%和6.4%,差异均有统计学意义(P<0.05)。结论相对于ELISA法,化学发光酶免疫分析法在丙型肝炎中的应用有较高的检出阳性率,对于相关抗体检测有较高的敏感性,值得推广应用。Objective To compare the effects of chemiluminescent enzyme immunoassay and ELISA method for the detection of hepatitis C antibodies. Methods 140 inpatients with hepatitis C in our hospital were selected and the blood samples were collected for detecting hepatitis C antibodies by adopting the chemiluminescence immunoassay and ELISA method. Results The positive detection rate of anti hepatitis C virus（HCV） antibody detected by the chemiluminescent enzyme immunoassay was 98.6 %,which was higher than 94.3 % detected by the ELISA method, the difference was statistically significant （P〈0.05）. The positive rates of chemiluminescent enzyme immunoassay for detecting anti AMA-M2 antibody, anti-3 E antibody, anti-SP100 antibody, anti-PML antibody and anti-GP210 antibody were 80.0% ,73.6% ,37.9% ,55.7% and 47.9%. respectively,while which detected by the ELISA method were 12.9 %, 12.9%, 13.6%, 10.7 % and 6.4 % respectively, the differences were statistically significant （P〈0.05）. Conclusion Compa.red with the ELISA method,the chemiluminescent enzyme immunoassay has very good positive detec- tion rate in hepatitis C, has better sensitivity for the relevant antibody detection, at the same time has higher detection sensitivity and is worthy of being promoted and applied.

关 键 词：化学发光酶免疫分析法 酶联免疫吸附试验 丙型肝炎病毒 抗体 

分 类 号：R512.62[医药卫生—内科学]