3种体液细胞染色方法的效果比较  

Comparison of 3 kinds of method for staining body fluid cells

作　　者：杨麦贵[1] 杨铮[2] 杨阳[3] 岳波[4] 郑善銮[1] 何娟[1] 樊爱琳[1] 郝晓柯[1] 

机构地区：[1]第四军医大学西京医院全军临床检验医学研究所,西安710032 [2]第四军医大学学员旅,西安710032 [3]第四军医大学西京医院全军整形外科研究所,西安710032 [4]第四军医大学西京医院耳鼻咽喉头颈外科,西安710032

出　　处：《检验医学与临床》2016年第1期53-54,共2页Laboratory Medicine and Clinic

摘　　要：目的选择简单、快速且能反映体液细胞原有结构特征的染色最佳方法。方法比较临床较为常用的苏木精-伊红(HE)染色法、巴氏染色法及瑞-姬(WG)混合染色法对650份不同体液标本进行细胞染色效果及阳性检出率。结果 WG混合染色法操作简便快速,能使体液细胞结构最大限度地保持原有状态及特征,细胞质、细胞核及核仁之间的色差大,易于区分辨认,阳性诊断符合率为99.7%、灵敏度为79.0%、总有效率达94.0%。结论经对各种体液细胞染色观察分析,WG混合染色法效果优于HE和巴氏染色法,特适用于各级检验单位体液细胞的开展。Objective To select a simple,rapid and best staining method enabling to reflect the original structure charactristics of body fluid cells. Methods The effects of cell staining and postive detection rates of 650 different body fluid specimens were compared among the clinically commonly used hematoxylin eosin （HE） staining,papanico- laou staining and Swiss-Ji （WG） mixed staining. Results The WG mixed staining was simple and rapid to operate, enabling the cell structure to maximize the body fluid cells and maintaining the original condition and characteristics brightly and clearly, the color difference was larger among cytoplasm, nucleus and nucleolus, easy to distinguish and i dentify, the positive accuracy was 99.7 %, the sensitivity was 79.0 % and the total effective rate reached 94.0 %. Conclusion After observing and analyzing the staining effect of various body fluid cells,the WG staining method has better effect than the HE staining and the Papanicolaou staining,which is especially suitable to conduct the detection of body fluid ceils at various levels of laboratories.

关 键 词：体液 苏木精-伊红染色法 巴氏染色法 瑞-姬混合染色法 

分 类 号：R446.19[医药卫生—诊断学]