大黄素对人肝癌SMMC7721细胞增殖的抑制作用  

Inhibitory Effects of Emodin on the Proliferation of Human Hepatocellular Carcinoma SMMC7721 Cells

作　　者：王昕雯[1] 朱国光[2] 

机构地区：[1]南京医科大学附属淮安第一医院,江苏淮安223300 [2]南京医科大学附属淮安第一医院一分院,江苏淮安223001

出　　处：《中国药房》2016年第1期58-60,共3页China Pharmacy

摘　　要：目的:研究大黄素对人肝癌SMMC7721细胞增殖的抑制作用。方法:以0(阴性对照)、25、37.5、50、62.5、75、87.5、100μmol/L大黄素溶液和100μmol/L 5-氟尿嘧啶(5-FU)培养SMMC7721细胞24、48、72 h,检测细胞光密度,计算增殖抑制率。以0(阴性对照)、25、50、75μmol/L大黄素溶液和100μmol/L 5-FU培养SMMC7721细胞48 h,检测细胞凋亡率、周期分布和细胞Bax、B淋巴细胞瘤2(Bcl-2)基因表达。结果:与阴性对照比较,25、37.5、50、62.5、75、87.5、100μmol/L大黄素溶液和100μmol/L 5-FU作用后细胞增殖抑制率升高,且与大黄素的浓度和作用时间呈正相关。与阴性对照比较,25、50、75μmol/L大黄素和100μmol/L 5-FU作用后细胞凋亡率升高、Bax基因表达增强、Bcl-2基因表达减弱,其中50、75μmol/L大黄素溶液和100μmol/L 5-FU能明显滞留细胞于G0/G1期(P<0.05或P<0.01)。结论:大黄素能抑制SMMC7721细胞增殖,促进其凋亡,阻止其生长。OBJECTIVE：To study the inhibitory effects of emodin on the proliferation of human hepatocellular carcinoma SMMC7721 cells. METHODS：SMMC7721 cells were treated with 0（negative control）,25,37.5,50,62.5,75,87.5,100 μmol/L emodin solution and 100 μ mol/L 5-FU for 24 h,48 h,72 h. The optical density value of cells was detected,and inhibition rate was calculated. SMMC7721 cells were treated with 0（negative control）,25,50,75 μ mol/L emodin solution and 100 μ mol/L5-FU for 48 h,and cell apoptosis rate,cell cycle and the expression of Bax and Bcl-2 gene were detected. RESULTS：Compared with negative control,the rate of cell proliferation inhibition increased after treated with 25,37.5,50,62.5,75,87.5,100 μmol/L emodin and 100 μmol/L 5-FU,which was positively associated with the concentration and duration. Compared with negative control,the rate of cell apoptosis increased after treated with 25,50,75 μmol/L emodin solution and 100 μmol/L 5-FU;the expression of Bax increased and that of Bcl-2 dereased;50,75 μmol/L emodin solution and 100 μmol/L 5-FU could arrested cells at G0/G1phase（P〈0.05 or P〈0.01）. CONCLUSIONS：Emodin can inhibit the proliferation of SMMC7721,promote cell apoptosis and inhibit cell growth.

关 键 词：大黄素 人肝癌SMMC7721细胞 BAX B淋巴细胞瘤-2 细胞周期 凋亡 

分 类 号：R965[医药卫生—药理学]