六株湖北省ALV-J地方分离株的gp85基因序列分析  

Analysis of gp85 Gene Sequences of 6 ALV-J isolates in Hubei Province

作　　者：汪宏才[1] 罗青平[1] 温国元[1] 张腾飞[1] 杨峻[1] 艾地云[1] 

机构地区：[1]湖北省农业科学院畜牧兽医研究所/动物胚胎工程及分子育种湖北省重点实验室,武汉430064

出　　处：《湖北农业科学》2015年第23期5956-5958,共3页Hubei Agricultural Sciences

基　　金：湖北省现代农业技术体系项目(NYTX-JQ-03);国家现代农业产业技术体系专项资金项目(CARS-42-G11);湖北省农业科技创新中心资助项目(2011-620-004-003);农业部公益行业科研专项(201303044;201303033)

摘　　要：通过对ALV-J病毒gp85基因进行分析,发现6个湖北分离株与国际标准的肉鸡分离毒株HPRS103株核苷酸序列相似性为93.9%~98.2%,氨基酸序列相似性为88.8%~97.8%。其中Hu B09XZ01株、Hu B09JY03株、Hu B09JY04株与HPRS103株核苷酸序列相似性达到98.1%~98.2%,氨基酸序列相似性达到97.4%~97.8%。系统遗传进化表明,湖北分离株Hu B09XZ01株、Hu B09JY03株、Hu B09JY04株与ALV-J国际标准毒株HPRS103遗传距离最近。通过分析表明,湖北省ALV-J主要来源于肉鸡,大都通过引种感染,这对于湖北省禽白血病的净化以及家禽品种的选育具有重要参考意义。Through the analysis of gp85 gene of ALV-J virus,the nucleotide sequences of 6 strains isolated from Hubei show93.9% ~98.2% similarity to the international standard strain HPRS103 isolated from broiler,the similarity of amino acid sequences range between in 88.8%~97.8%. Hereinto,The similarity of nucleotide sequence and amino acid sequence between strains Hu B09XZ01,Hu B09JY03,Hu B09JY04 and the international standard strain HPRS103 reached 98.1%~98.2% and97.4%~97.8% respectively.Phylogenetic tree showed that,Hubei isolates Hu B09XZ01,Hu B09JY03 and Hu B09JY04 were close to HPRS103.These results suggested that ALV-J in Hubei province mainly comes from broiler,and has the reference significance to poultry breeding in our province.

关 键 词：禽白血病 J亚群 相似性 进化树 

分 类 号：S858.31[农业科学—临床兽医学]