溶出伏安法检测尿碘的方法学评价  

Methodological evaluation on stripping voltammetry for urinary iodine determination

作　　者：许中[1] 盛青松 张同军 辛君伟 

机构地区：[1]苏州市立医院东区同位素室,江苏苏州215001 [2]无锡市申瑞生物制品有限公司,江苏无锡214092 [3]江苏省丝网印刷电极式生物传感器工程技术研究中心,江苏无锡214092

出　　处：《检验医学》2015年第12期1226-1228,共3页Laboratory Medicine

基　　金：江苏省自然科学基金资助项目(BK2012539)

摘　　要：目的验证溶出伏安法测定尿碘的临床应用性能。方法采用SR-I-100尿碘专用型微量元素检测仪(溶出伏安法)测定人尿冻干粉和尿临床样本,验证溶出伏安法的正确度、精密度、线性、交叉污染、最低检测限,并与过硫酸铵消化-砷铈催化分光光度法进行比较。结果溶出伏安法测定低(104μg/L)、高(486μg/L)浓度人尿冻干粉的相对偏倚分别为-1.76%和3.62%;测定低(80.89μg/L)、中(210.33μg/L)、高(538.50μg/L)3个浓度尿样本的变异系数(CV)分别为9.81%、4.27%、3.64%;尿碘浓度在100~540μg/L时检测呈线性,线性回归方程为Y=88.525X-6.678 0,R^2=0.993 1;样本间不存在交叉污染;检测低限为50μg/L(CV=12.85%)。溶出伏安法与过硫酸铵消化-砷铈催化分光光度法的测定结果差异无统计学意义(P>0.05),且相关性良好(R^2=0.97)。结论溶出伏安法测定尿碘有良好的正确度、精密度、线性和检测限,不存在交叉污染,可在临床上推广应用。Objective To evaluate the performance of stripping voltammetry for urinary iodine determination.Methods The trueness,precision,linear,cross-contamination and detection limit were analyzed by measuring human urinary freeze-dried powder and urine samples by stripping voltammetry using SR-I-100 microelement analyzer for urinary iodine,and the results were compared with those of arsenic cerium catalytic spectrophotometry. Results By stripping voltammetry,the biases of human urinary freeze-dried powders in low and high urinary iodine levels（ 104 μg / L and 486μg / L） were- 1. 76% and 3. 62%. The coefficients of variation（ CV） for low,medium and high levels of urine samples（ 80. 89 μg/L,210. 33 μg/L and 538. 50 μg/L） were 9. 81%,4. 27% and 3. 64%. When the linear range was 100-540 μg / L,the equation of linear regression was Y = 88. 525X- 6. 678 0,R^2= 0. 993 1. There was no crosscontamination. The lowest detection limit was 50μg / L. The CV was 12. 85%. Between arsenic cerium catalytic spectrophotometry and stripping voltammetry,there was no statistical significance（ P 〉 0. 05）,and the correlation was good（ R^2= 0. 97）. Conclusions The stripping voltammetry has good trueness,precision,linear and detection limit without cross-contamination,and is suitable for clinical application.

关 键 词：溶出伏安法 碘 尿液 芯片传感 砷铈催化分光光度法 

分 类 号：R446.1[医药卫生—诊断学]