电感耦合等离子体质谱检测人全血中的汞  

Determination of mercury in whole blood by inductively coupled plasma-mass spectrometry

作　　者：刘军霞[1] 周昕[1] 颜崇淮[1] 

机构地区：[1]上海交通大学医学院附属新华医院环境试验室,上海200092

出　　处：《检验医学》2015年第12期1229-1233,共5页Laboratory Medicine

基　　金：环境保护部环保公益性项目(201309048);科技部973课题(2012CB525001)

摘　　要：目的研究电感耦合等离子体质谱法(ICP-MS)检测全血中汞的可行性。方法采用盐酸作为样本基质,用微波消解仪对全血进行消解,然后用ICP-MS检测全血汞,验证ICP-MS的检测下限、功能灵敏度、分析测量范围、回收率、准确度和精密度。结果 ICP-MS的检出限为4.525×10^(-2)ng/mL,功能灵敏度为1.495 ng/mL,分析测量范围为0.18~13.83 ng/mL,回收率为92%~103%。低值[(20±5)ng/mL]和高值[(50±8)ng/mL]质控品20 d检测均值分别为22.22和51.39 ng/mL,批间精密度分别为6%和5%;1 d内检测临床随机混合全血、低值和高值全血质控品各20份,批内精密度分别为4.8%、4.8%、4.4%。结论 ICP-MS检测全血汞可以满足临床要求。Objective To study the feasibility of inductively coupled plasma-mass spectrometry（ ICP-MS） for whole blood mercury determination. Methods Using hydrochloric acid as sample matrix,samples were digested with microwave digestion instrument,and ICP-MS was used to determine mercury in whole blood. The lowest detection limit,functional sensitivity,range,recovery,accuracy and precision of ICP-MS were evaluated. Results The detection limit of this method was 4. 525 × 10-2ng / mL,the functional sensitivity was 1. 495 ng / mL,the analytical measurement range was 0. 18-13. 83 ng / mL,and the recovery rate was 92%-103%. The average values of low-level [（ 20 ± 5） ng / mL ]and high-level [（ 50 ± 8） ng / m L] quality control materials for 20 d were 22. 22 ng / mL and 51. 39 ng / mL,and between-run precisions were 6% and 5%. Clinical random mixed whole blood,low and high whole blood quality control materials for 20 parts each were detected within 1 d,and the within-run precisions were 4. 8%,4. 8% and 4. 4%.Conclusions ICP-MS for the determination of whole blood mercury meets the clinical requirements.

关 键 词：电感耦合等离子体质谱 汞 全血 

分 类 号：R446.1[医药卫生—诊断学]