稳定表达GFPu蛋白的人前列腺癌PC-3细胞系构建及荧光图像的定量分析  

Construction of Human Prostatic PC-3 Cell Line with Stably Expressing GFPu and Quantitative Analysis of its Fluorescence Images

作　　者：陈彦利[1] 陈慧甜 梁湘如 胡雨馥 时国庆[1] 刘杰民[1] 

机构地区：[1]北京科技大学化学与生物工程学院,北京100083

出　　处：《齐鲁工业大学学报》2015年第3期1-6,共6页Journal of Qilu University of Technology

基　　金：国家高技术研究发展计划(2012AA101609-5);新世纪优秀人才支持计划(NCET-11-0581)

摘　　要：泛素蛋白酶体系统是广受关注的抗肿瘤药物靶标,细胞内蛋白酶体活性变化的实时检测对抗肿瘤药物筛选及作用机理研究具有重要意义。本文构建了稳定表达GFPu蛋白的人前列腺癌PC-3细胞系,并建立了对GFPu蛋白荧光图像的定量分析方法。该方法可以在不影响细胞正常生命过程,不加入荧光底物的条件下,实现对细胞内蛋白酶体活性变化的实时、动态、定量监控。The ubiquitin-proteasome systemis a widely concerned target of anticancer drugs. Realtime detection of intracellular proteasome activity is of great significance for the screening and mechanismstudy of anticancer drugs.In this paper,Human prostate cancer PC- 3 cell lines which stably expressing GFPu was constructed.Furthermore,a quantitative analysis method for the GFPu fluorescence images had been established,which can realize real-time,dynamic,quantitative monitoring of proteasome activity change without affecting the normal cell life process or adding of fluorescent substrate.

关 键 词：泛素蛋白酶体系统 GFPu蛋白 PC-3细胞 蛋白酶体活性 实时监测 

分 类 号：Q5-3[生物学—生物化学]