不同pH值和活性炭浓度对蓝莓不定芽分化的影响  

Effects of pH and Active Carbonon the Vaccinium Spp Tissue Culture

作　　者：张文泉[1] 何志国[2] 杨秀钟 袁继林 

机构地区：[1]凯里学院,贵州凯里556011 [2]国家林业局中南林业调查规划设计院,长沙410014 [3]黔东南州林业局,贵州凯里556000

出　　处：《中南林业调查规划》2015年第3期55-57,共3页Central South Forest Inventory and Planning

基　　金：黔东南州自然科学项目(黔东南科合J字[2014]4001);凯里学院博士专项课题(凯里学院科同BS201339)

摘　　要：以MS培养基作为基础培养基,采用蓝莓当年生的幼嫩枝条为实验材料,研究在不同pH值(5.0,5.1,5.2,5.3,5.4,5.5)和不同活性炭浓度(0.01%,0.03%,0.05%,0.1%,0.2%)下对蓝莓不定芽分化的影响。试验结果表明:蓝莓不定芽分化最佳的pH值条件为5.0,最适宜的活性炭浓度为0.1%。Use the MS medium as basic medium,this paper adopted the Vaccinium Spp as experimental materials,exploration on how the different pH（ 5. 0,5. 1,5. 2,5. 3,5. 4,5. 5） and different concentrations of active carbon（ 0. 01%,0. 03%,0. 05%,0. 1%,0. 2%） affect the Vaccinium Spp tissue culture. Experimental results showed that with pH 5. 0 and activated carbon concentration 0. 1%,the Vaccinium Spp callus induction and adventitious bud differentiation were the best.

关 键 词：蓝莓 不定芽 PH值 活性炭 

分 类 号：S723[农业科学—林木遗传育种]