白粉菌侵染中国月季对甜菜夜蛾幼虫乙酰胆碱酯酶活性的影响  

Effects of Infection of Rose Plants by Rose Powdery Mildew on Activity of Acetylcholinesterase of Larvae of Beet Armyworm

作　　者：杨发忠[1] 杨德强[2] 杨斌[1] 孙浩[1] 

机构地区：[1]西南林业大学云南省森林灾害预警与控制重点实验室,云南昆明650224 [2]云南师范大学信息学院,云南昆明650092

出　　处：《河南农业科学》2015年第12期75-78,共4页Journal of Henan Agricultural Sciences

基　　金：国家自然科学基金项目(31160354);云南省教育厅科学研究基金项目(2013Y120)

摘　　要：采用光度法研究中国月季感染白粉菌对甜菜夜蛾幼虫体内蛋白质含量和乙酰胆碱酯酶(AChE)活性的影响,为寄主植物介导的间接的病虫互作机制研究提供依据。结果表明,中国月季感染月季白粉菌后对取食中国月季的甜菜夜蛾产生了不利影响,使幼虫蛋白质含量和乙酰胆碱酯酶活性降低。取食感病中国月季叶片9 d后,与取食健康叶片相比,幼虫体内蛋白质含量下降了81.77%(P<0.01),同时,AChE比活力为8.89μmol/(mg·min),下降了68.03%(P<0.01)。AChE的米氏常数(Km)和最大反应速率(v_(max))也受到明显影响,Km增大(P<0.001)、v_(max)减小(P<0.001),均表明AChE活性降低。从昆虫生理学角度证明,白粉菌侵染中国月季对甜菜夜蛾造成的抑制作用与幼虫体内蛋白质含量变化和AChE活性变化有关。With spectrometry method,the changes of protein content and acetylcholinesterase（ AChE） activity in the larvae of beet armyworm,Spodoptara exigua（ Hübner）,fed on rose leaves infected with rose powdery mildew,Sphaerotheca pannosa（ Wallr. ： Ex Fr.） Lév.,were determined in the present study,to provide reference for understanding the mechanism of plant-mediated indirect interaction between phytopathogenic fungi and insects. The beet armyworm was detrimentally influenced by infection of its host plants of rose,Rosa chinensis Jacq. Therefore,the protein content and the AChE activity of the larvae fed on mildew-infected rose leaves decreased. After the larvae were fed on infected rose leaves for nine days,the protein content decreased by 81. 77%（ P〈 0. 01） and the AChE activity[8. 89 μmol/（ mg·min） ]decreased by 68. 03%（ P 〈0. 01）,when compared with larvae fed on healthy rose leaves. Also the Km（ Michaelis-Menten constant） and v_（max）（ maximum velocity of hydrolysis） of AChE were detrimentally affected[Kmincreased（ P 〈0. 001） and v_（max）decreased（ P 〈0. 001） ],showing that the AChE activity decreased when the larvae were fed with mildew-infected rose leaves. The obtained results from the aspect of insect physiology demonstrate that the inhibition of infection of rose plants by powdery mildew on beet armyworm is clearly related to the changes of protein content and activity of AChE in the larvae of beet armyworm.

关 键 词：甜菜夜蛾 中国月季 白粉菌 乙酰胆碱酯酶 病虫互作 

分 类 号：S436.8[农业科学—农业昆虫与害虫防治]