HPLC法测定茯苓配方颗粒中茯苓酸的含量  

Determination of content of pachymic acid in poria formula granule by HPLC

作　　者：周军[1] 史德胜 王杰[1] 刘冰[1] 邵建强[1] 刘雪莹 

机构地区：[1]天津市药品检验所,天津300070 [2]天津丹溪国药研究所,天津300061 [3]天津市市场和质量监督管理委员会,天津300070

出　　处：《天津药学》2015年第6期16-17,共2页Tianjin Pharmacy

基　　金：天津市科技支撑计划重点项目(No.12ZCZDSY01000)

摘　　要：目的：建立高效液相色谱法测定茯苓配方颗粒中茯苓酸的含量。方法：采用HPLC法,乙腈-0.5%磷酸溶液（83∶17）为流动相,流速为1.0 ml/min,检测波长为210 nm,柱温为35℃。结果：6个厂家12批次的茯苓配方颗粒中茯苓酸含量介于0.87-69.13μg/g之间。结论：该方法操作简便,结果稳定可靠,可用于茯苓配方颗粒的质量评价。Objective： To establish an HPLC method for determination of pachymic acid in poria formula granule. Methods：HPLC was performed on a Diamonsil C18（ 4. 6 mm × 250 mm,5 μm） column at 35 ℃ with mobile phase consisting of acetonitrile-0. 5% phosphoric acid solution（ 83∶ 17）. The flow rate was 1. 0 ml / min. The detection wavelength was 210 nm. Results： The content of pachymic acid in twelve batch es of poria formula granule products from six factories ranged between 0. 87 μg / g and69. 13 μg / g. Conclusion： The method is simple and reliable,and can be used for the quality evaluation of poria formula granule.

关 键 词：茯苓配方颗粒 HPLC 茯苓酸 

分 类 号：R927.2[医药卫生—药学]