哮喘患儿血清炎性介质变化及临床意义  

Changes of serum inflammatory mediators in children with asthma and its clinical significance

作　　者：张宏[1] 王丙增[1] 张旭亚[1] 况琦[1] 孟凡威[1] 付晓梅[1] 

机构地区：[1]河南大学第一附属医院儿科,河南开封475001

出　　处：《中国中西医结合儿科学》2015年第6期560-562,共3页Chinese Pediatrics of Integrated Traditional and Western Medicine

基　　金：2012年度河南省医学学术技术带头人出国培训计划项目(201204158)

摘　　要：目的测定哮喘儿童血清生长因子β_1(TGF-β_1)、Ⅰ型纤溶酶原激活物抑制因子(PAI-1)、基质金属蛋白酶-9(MMP-9)的表达变化,探讨TGF-β_1、PAI-1、MMP-9在儿童支气管哮喘呼吸道重塑的作用机制。方法选取符合支气管哮喘诊断的患儿40例,给予布地奈德雾化吸入治疗,采集患儿血清检测治疗前后TGF-β_1、PAI-1、MMP-9水平变化,同时观测肺功能的变化,并与对照组(健康儿童)比较。结果经测定哮喘患儿血清样本中的TGF-β_1、PAI-1、MMP-9含量均明显增高,与对照组比较,差异有统计学意义(P<0.05);同时测的FEV1、PEF的百分比均降低;观察组给予布地奈德雾化吸入治疗后,哮喘患儿血清样本中的GF-β_1、PAI-1、MMP-9含量逐渐下降,与治疗前比较,差异有统计学意义(P<0.05);同时测的FEV1、PEF的百分比与治疗前比较显著好转,差异有统计学意义(P<0.01)。结论 TGF-β_1、PAI-1、MMP-9水平变化与儿童支气管哮喘有重要的相关性;布地奈德雾化吸入治疗能明显改善患儿的呼吸情况,其机制可能是通过降低TGF-β_1、PAI-1、MMP-9的水平而抑制气道重塑以及气道炎症,达到治疗哮喘的目的。Objective To determine the expression of TGF-β_1,PAI-1and MMP-9,and to study their mechanism of action in bronchial asthma airway remodeling in children.Methods Forty patients with bronchial asthma in children were given budesonide atomization inhalation treatment and serum were collected and detected before and after treatment for TGF-β_1,PAI-1and MMP-9levels.At the same time we observed changes in lung function,and compared with the control group（healthy children）.Results In the serum samples of children with asthma the TGF-β_1,PAI-1and MMP-9content increased significantly（compared with the control group）,and the differences were statistically significant（P〈0.05）;at the same time,percentage of FEV1 and PEF decreased;after budesonide inhalation treatment,TGF-β_1,PAI-1and MMP-9content decreased,and compared with before treatment,there were significant statistical differences（P〈0.05）;the percentage of FEV1 and PEF was significantly improved compared with those before treatment,the difference being statistically significant（P〈0.01）.Conclusion PAI-1,MMP-9and TGF-β_1changes in children are closely related to bronchial asthma;budesonide inhalation therapy can significantly improve the respiratory condition of the patients,and the possible mechanism may be by reducing the TGF-β_1,PAI-1and MMP-9level to ihibit airway remodeling and airway inflammation,and achieve the purpose of treating asthma.

关 键 词：哮喘 TGF-β_1 PAI-1 MMP-9 气道重塑 儿童 

分 类 号：R725.622.5[医药卫生—儿科]