番茄灰霉生防菌CQ的分子鉴定及其生防效果研究  

Screening, Identification and Biocontrol Effects of Antagonistic Bacterial Strain CQ against Botrytis cinerea from Tomato

作　　者：杨利敏[1,2] 仝赞华[2] 郭立华[2] 蒋细良[2] 邱德文[2] 李淼[1,3] 

机构地区：[1]安徽农业大学植物保护学院/安徽省农产品安全重点实验室,合肥230036 [2]中国农业科学院植物保护研究所/农业部生物防治重点开放实验室,北京100081 [3]中国科学院植物研究所/中国科学院北方资源植物重点实验室,北京100093

出　　处：《中国生物防治学报》2015年第6期956-960,共5页Chinese Journal of Biological Control

基　　金：公益性行业(农业)科研专项(201403025);植物病虫害生物学国家重点实验室开放基金(SKL2009OP10);中国博士后科学基金(20100470108);安徽省自然科学基金(1408085MC68)

摘　　要：菌株CQ是一株分离自云南省保山市昌宁县烟草根部的对番茄灰霉病具有较好防治效果的拮抗细菌。通过对该菌株进行形态特征观察、16S r DNA序列分析鉴定,确定菌株CQ为枯草芽孢杆菌Bacillus subtilis。拮抗试验结果表明,菌株CQ能够显著抑制番茄灰霉菌的菌丝生长,使菌丝出现异常分枝和囊泡状畸形。1‰CQ发酵滤液即可显著抑制番茄灰霉菌孢子的形成。室内生测结果表明,相比对照组,48 h无菌发酵滤液原液在离体叶片上对番茄灰霉病的防效可达100%,而其10、100倍稀释滤液则使发病率分别降低28.5%和21.3%,病情指数分别降低44.01和37.86。Bacteria strain CQ was isolated from Yunnan Provence, which exhibited a strong antagonistic activity on mycelia growth of Botrytis cinerea. Affected fungal hyphae formed abnormal branches and bubble deformation structures. The formation of the spores of B. cinerea was inhibited in agar medium amended with 1‰ cultural filtrate of the strain. Control efficacies on tomato gray mold disease caused by B. cinerea were 100%, when detached tomato leaves were sprayed with 1‰ CQ cultural filtrate of the strain; the incidence decreased by 28.5%, 21.3%, and the disease index decreased by 44.01, 37.86 respectively, when using its 10 times and 100 times dilution filtrate sprayed on tomato seedlings. The strain CQ was identified as Bacillus subtilis according to its biochemical characteristics.

关 键 词：灰葡萄孢菌 枯草芽孢杆菌 鉴定 生防效果 

分 类 号：S476[农业科学—农业昆虫与害虫防治]