荧光共振能量转移探针检测药物中单磷酸腺苷的方法研究  

Development of Fluorescence Resonance Energy Transfer Sensor for Determination of Adenosine Monophosphate in Biological Drug

作　　者：董玲玉[1] 杜宏明[1] 王鹏[1] 王立云[1] 李一珂 翟红[1] 冯婷[1] 王香凤[1] 朱桥友 谢孟峡[1] 

机构地区：[1]北京师范大学分析测试中心,北京100875

出　　处：《光谱学与光谱分析》2015年第11期3096-3099,共4页Spectroscopy and Spectral Analysis

基　　金：国家科技支撑计划(2012BAK26B04);锦州奥鸿药业有限责任公司项目资助

摘　　要：小牛血清去蛋白药物能够促进缺氧细胞对氧与葡萄糖的摄取和利用,改善外周及脑部的血液循环障碍和营养失调,促进烧伤创伤愈合,调节中枢神经系统等诸多药理药效。单磷酸腺苷(AMP)为该药物的活性成份之一,构建了以β-环糊精(β-CD)修饰的硫化锌量子点为能量供体,3-羟基黄酮为能量受体的能量共振转移荧光探针。研究发现AMP能够引起该探针在526nm处的荧光发射峰强度的猝灭,并且AMP的浓度与探针的荧光强度的Stern-Volmer曲线呈现良好的线性关系,线性相关系数为0.996,该探针能够应用于药品中AMP的检测。The biological drug of the calf-blood dialysate has various pharmacological effects.It can promote the oxygen and glucose uptake for the hypoxia cells,and has beneficial effects on the malfunction of the blood circulation and trophic disturbances in the brain,and the impairment of peripheral blood circulation.Furthermore,it is favorable to wound healing and can regulate the central nervous system.Adenosine monophosphate（AMP）is a main active ingredient of the biological drug.In this report,a fluorescence resonance energy transfer（FRET）sensor has been developed withβ-CD-capped ZnS QDs as energy donor and 3-hydroxyflavone（3-HF）as energy acceptor.The results showed that AMP can lead to the fluorescence quenching of the FRET sensor at 526 nm,and the Stern-Volmer curve between the fluorescence quenching and the concentrations of AMP present a satisfactory linearity with the correlation coefficient of 0.996.The developed sensor has successfully applied for determination of the AMP in the biological drug.

关 键 词：能量共振转移探针 β-CD-ZnS量子点 小牛血清去蛋白药物 单磷酸腺苷 

分 类 号：O657.3[理学—分析化学]