新疆野苹果(Malus sieversii)内生菌的分离与鉴定  

Isolation and Identification of Endophytic Fungi in Malus sieversii

作　　者：李芳[1] 邹妍[1] 刘彬[1] 张云秀[1] 龙鸿[1] 阎国荣[1] 

机构地区：[1]天津农学院园艺园林学院,天津300384

出　　处：《天津农学院学报》2015年第4期1-5,共5页Journal of Tianjin Agricultural University

基　　金：新疆维吾尔自治区财政林业科技专项项目"新疆野苹果种质资源收集与评价"(无编号)

摘　　要：采用组织分离法从新疆野苹果茎中分离纯化了内生菌纯菌株,用分子生物学方法对内生菌进行了种属鉴定,扩增了真菌ITS序列,并进行了比较分析。结果显示,从新疆野苹果中分离得到了11株内生菌,分属于2个属3个种,分别为链格孢属(Alternaria sp.)和镰刀菌属(Fusarium sp.),新疆野苹果与枣树、棕榈树和甘蔗等有相同种类的内生菌,结合MS培养基启动培养、PDA法分离可快速得到纯菌株,比PDA多次纯化分离法更简便高效。本文可为抑制新疆野苹果组织培养时产生的内生菌的生长提供参考。The endophytic fungi was isolated from M. sieversii stem using tissue separation method to obtain pure strains endophytes, the endophytic fungi species was identified using the molecular biology method, and the fungi ITS sequence was amplified and the comparation and analyses were made. The results show that 11 strains of endogenous fungi were identified from M. sieversii, which belonged to 3 species of endophytes in two genera（Alternaria sp. and Fusarium sp. ）, and there are same kind of endophytes among jujube, palm trees, M. sieversii and sugar cane. Pure strains can be obtained in short time initiated with MS medium and separated by PDA method. This method is simpler and more effective compared with multiple purified isolation method of PDA. These can provide experimental data for inhibiting the growth of endophytes generated from M. sieversii by the tissue culture.

关 键 词：新疆野苹果 内生菌 组织分离法 ITS 

分 类 号：S661.1[农业科学—果树学]