近红外光谱法测定老芒麦营养价值  

NIRS for Predicting Nutritive Value of Elymus Sibiricus

作　　者：严旭[1,2,3] 白史且[1,2] 鄢家俊[1] 胡超[4] 李达旭[1] 游明鸿[1] 张玉[1] 刀志学[4] 张昌兵[1] 干友民[2] 

机构地区：[1]四川省草原科学研究院,四川成都611731 [2]四川农业大学草学系,四川雅安625014 [3]四川省农业科学院蚕业研究所,四川南充637000 [4]西南民族大学生命科学与技术学院,四川成都610041

出　　处：《光谱学与光谱分析》2015年第8期2103-2107,共5页Spectroscopy and Spectral Analysis

基　　金：国家"十二五"科技支撑项目(2012BAD13B06)资助

摘　　要：老芒麦（Elymus sibiricus L.）是青藏高原地区主要禾本科牧草,对该区畜牧业具有重要作用。粗蛋白（CP）、酸性洗涤纤维（ADF）、中性洗涤纤维（NDF）和体外干物质消化率（IVDMD）是影响牧草营养价值高低的主要参数。基于近红外光谱技术（NIRS）结合偏最小二乘法建立了老芒麦CP,ADF,NDF和IVDMD的近红外预测模型。所建CP,ADF,NDF和IVDMD模型校正决定系数（R2cal）分别为0.994 5,0.949 9,0.9133和0.982 2,校正标准差（SEC,%DM）分别为0.329 9,0.779 9,1.343 0和1.376 2;验证决定系数（R2val）分别为0.993 8,0.944 9,0.890 7和0.979 0,验证标准差（SEP,%DM）分别为0.362 1,0.787 8,1.385 2和1.430 3。预测相关系数（r）大于0.943 8,相对分析误差（RPD）为3.02～12.63,表明NIRS能够对老芒麦CP,ADF,NDF和IVDMD进行准确分析。Siberian wildrye（Elymus sibiricus L.）is one of the predominant pasture species in Qinghai-Tibet plateau of China.It supplies a large number of fodders to domestic animals in spring and winter,and provides a large proportion of the summer and autumn grazing in these alpine regions.Crude protein（CP）,acid detergent fiber（ADF）,neutral detergent fiber（NDF）and in vitro dry matter digestibility（IVDMD）are the most important aspects of nutritive value of forages.A successful application of near infrared spectroscopy（NIRS）in combination with partial least square regression（PLSR）for the determination of four parameters（CP,ADF,NDF and IVDMD）of Siberian wildrye was developed.The standard errors of calibration（SEC,%DM）and R2calvalues（in parentheses）were 0.329 9（0.994 5）,0.779 9（0.949 9）,1.343 0（0.913 3）,and 1.376 2（0.982 2）for CP,ADF,NDF and IVDMD equations,respectively.The standard errors of prediction（SEP,%DM）and R2 val values（in parentheses）were 0.362 1（0.993 8）,0.787 8（0.944 9）,1.385 2（0.890 7）,and 1.430 3（0.979 0）for CP,ADF,NDF and IVDMD,respectively.A good correlation（r〉0.943 8）was found between results from NIRS and the traditional chemical method,and residual predictive deviation（RPD）ranged from 3.02 to 12.63.It was concluded that NIR spectroscopic technique associated with chemometrics is sufficiently sensitive to allow the accurate prediction of the concentrations of components（CP,ADF and NDF）and IVDMD of Siberian wildrye.

关 键 词：近红外光谱法 老芒麦 粗蛋白 中性洗涤纤维 体外干物质消化率 

分 类 号：O657.3[理学—分析化学]