Spectrophotometric Methods for Simultaneous Determination of Amlodipine Besylate and Atenolol in Their Tablet Dosage Form  

作　　者：Nesrine T.Lamie 

机构地区：[1]Analytical Chemistry Department,Faculty of Pharmacy,Cairo University

出　　处：《光谱学与光谱分析》2015年第12期3538-3543,共6页Spectroscopy and Spectral Analysis

摘　　要：Three simple,specific,accurate and precise spectrophotometric methods are developed for simultaneous determination of amlodipine besylate(AM)and atenolol(AT)in tablets.The first method is dual wavelength spectrophotometry(DW).The second method is ratio subtraction(RS)which depends on subtraction of the plateau values from the ratio spectrum,coupled to first derivative of ratio spectra(1 DD).The third method applies bivariate calibration method using 210and 225nm as an optimum pair of wavelength for amlodipine and atenolol.The calibration curves are linear over the concentration range of 4~40μg·mL-1 for both drugs.The specificity of the developed methods is investigated by analyzing laboratory prepared mixtures of the two drugs and their combined dosage form.The two methods are validated as per ICH guidelines and can be applied for routine quality control testing.Three simple,specific,accurate and precise spectrophotometric methods are developed for simultaneous determination of amlodipine besylate（AM）and atenolol（AT）in tablets.The first method is dual wavelength spectrophotometry（DW）.The second method is ratio subtraction（RS）which depends on subtraction of the plateau values from the ratio spectrum,coupled to first derivative of ratio spectra（1 DD）.The third method applies bivariate calibration method using 210and 225nm as an optimum pair of wavelength for amlodipine and atenolol.The calibration curves are linear over the concentration range of 4~40μg·mL-1 for both drugs.The specificity of the developed methods is investigated by analyzing laboratory prepared mixtures of the two drugs and their combined dosage form.The two methods are validated as per ICH guidelines and can be applied for routine quality control testing.

关 键 词：AMLODIPINE ATENOLOL RATIO SUBTRACTION Dual wavelength Derivative-ratio BIVARIATE Spectro-photometry 

分 类 号：O657.3[理学—分析化学]