Impacts of X-ray irradiation on Saccharomyces cerevisiae cells growth and physiological-biochemical characteristic  

作　　者：曹国珍 张苗苗 李文建 缪建顺 陆栋 张文德 

机构地区：[1]Institute of Modern Physics, Chinese Academy of Sciences [2]School of Pharmacy, Lanzhou University [3]Key Laboratory of Microbial Resources Exploitation and Application [4]Gansu Ronghua Industry Group Co. Ltd

出　　处：《Nuclear Science and Techniques》2015年第6期46-54,共9页核技术（英文）

基　　金：Supported by the project of western talent training program of Chinese Academy of Sciences(No.Y306010XB0)

摘　　要：In this paper, the growth curves of yeast cells exposed to X-rays were detected, and then fitted by Gompertz equation. The yeast cells treated with 50–125 Gy showed an increased exponential growth rate, and lower total biomass at plateau. At doses ≥ 150 Gy, cells showed a decreased exponential growth rate and higher total biomass at plateau. DNA lesions were detected by comet assay. Meanwhile, intracellular accumulation of reactive oxygen species(ROS), reduction of mitochondrial membrane potential(?Ψ m) and cell membrane integrity were evaluated. We conclude that X-ray irradiation results in DNA lesions, ROS accumulation and?Ψ m decline in a dose-dependent manner, and that these changes may be one of causes of X-rays-induced apoptosis in yeast. Furthermore, yeast cell membrane integrity appeared compromised following irradiation,suggesting that membrane damage may also have a role in the biological effects of radiation.In this paper, the growth curves of yeast cells exposed to X-rays were detected, and then fitted by Gompertz equation. The yeast cells treated with 50–125 Gy showed an increased exponential growth rate, and lower total biomass at plateau. At doses ≥ 150 Gy, cells showed a decreased exponential growth rate and higher total biomass at plateau. DNA lesions were detected by comet assay. Meanwhile, intracellular accumulation of reactive oxygen species（ROS）, reduction of mitochondrial membrane potential（？Ψ m） and cell membrane integrity were evaluated. We conclude that X-ray irradiation results in DNA lesions, ROS accumulation and？Ψ m decline in a dose-dependent manner, and that these changes may be one of causes of X-rays-induced apoptosis in yeast. Furthermore, yeast cell membrane integrity appeared compromised following irradiation,suggesting that membrane damage may also have a role in the biological effects of radiation.

关 键 词：细胞生长曲线 X射线照射 酿酒酵母 生理生化特性 GOMPERTZ 线粒体膜电位 脱氧核糖核酸 试验检测 

分 类 号：Q937[生物学—微生物学] O434.1[机械工程—光学工程]