A micro E-DNA sensor for selective detection of dopamine in presence of ascorbic acid  

作　　者：朱丹 李敏 王丽华 左小磊 

机构地区：[1]Shanghai Institute of Applied Physics, Chinese Academy of Sciences [2]University of Chinese Academy of Sciences

出　　处：《Nuclear Science and Techniques》2015年第6期115-119,共5页核技术（英文）

基　　金：Supported by the National Natural Science Foundation of China(Nos.31470960 and 21422508)

摘　　要：In this paper, a novel method for selectively detection of dopamine(DA) in the interference of ascorbic acid(AA) is described. A nanometer-sized gold flower microelectrode(NGFME) is prepared by flame-etching and electrochemical deposition. The electrode tip was characterized by scanning electron microscope(SEM). The NGFME is sized at about 100 μm and dimensions of thorns of the electrode were in nanometers. By modifying with DA aptamer on the surface, the prepared aptasensor can selectively detect DA even in the presence of high concentration AA. Experimental results show that this NGFME has no response to AA. As a comparison, the carbon fiber electrode without DA aptamer modification is unable to effectively detect DA in the presence of AA. The NGFME is easy-to-prepare, selective and sensitive for DA detection down to 25 μM. The electrode can be expected to detect DA in vivo and in real biological samples.In this paper, a novel method for selectively detection of dopamine（DA） in the interference of ascorbic acid（AA） is described. A nanometer-sized gold flower microelectrode（NGFME） is prepared by flame-etching and electrochemical deposition. The electrode tip was characterized by scanning electron microscope（SEM）. The NGFME is sized at about 100 μm and dimensions of thorns of the electrode were in nanometers. By modifying with DA aptamer on the surface, the prepared aptasensor can selectively detect DA even in the presence of high concentration AA. Experimental results show that this NGFME has no response to AA. As a comparison, the carbon fiber electrode without DA aptamer modification is unable to effectively detect DA in the presence of AA. The NGFME is easy-to-prepare, selective and sensitive for DA detection down to 25 μM. The electrode can be expected to detect DA in vivo and in real biological samples.

关 键 词：选择性检测 DNA传感器 抗坏血酸 多巴胺 扫描电子显微镜 电化学沉积 碳纤维电极 电极尺寸 

分 类 号：TP212[自动化与计算机技术—检测技术与自动化装置] Q503[自动化与计算机技术—控制科学与工程]