γ刀照射三叉神经的剂量-效应关系研究  

The dose-effect relationship after the exposure of trigeminal nerve to gamma knife irradiation

作　　者：陈樵[1] 张曼[2] 李立东[3] 扈启宽[2] 李美育[2] 杜吉祥[1] 郭裕天[4] 刘晓冬[1] 

机构地区：[1]吉林大学公共卫生学院卫生部放射生物学重点实验室,长春130021 [2]宁夏医科大学生理教研室 [3]北京大学医学部干细胞研究中心 [4]深圳惠恒集团医学部

出　　处：《中华放射医学与防护杂志》2015年第12期904-909,共6页Chinese Journal of Radiological Medicine and Protection

基　　金：国家自然科学基金（31370837）

摘　　要：目的 探讨伽玛刀不同剂量照射大鼠三叉神经（感觉根）后对照射范围内三叉神经组织的损伤以及对周围临近脑干组织和脑桥的影响,为优化伽玛刀照射剂量提供理论依据.方法 选用成年雄性SD大鼠65只,分别以不同照射剂量（20、40、80、160 Gy）照射三叉神经,观察照射后1周、2周、4周大鼠行为学变化,取全脑组织进行HE、Nissl、MAPLC3B、53BP1、XRCC1染色观察细胞形态学和自噬以及DNA损伤和修复等变化.结果 所有剂量组照射大鼠均存活.20和40 Gy组均无行为学和病理学改变;80和160 Gy组均出现了照射部位毛发脱落;HE、Nissle、LC3B、53BP1及XRCC1染色发现,受照部位组织在80和160 Gy照射后,可见神经元细胞固缩、膨胀,神经元细胞发生自噬以及DNA损伤明显,160 Gy组DNA损伤明显加重.40 Gy照射后,神经元发生DNA损伤修复的程度较其他剂量增高.结论 伽玛刀照射三叉神经根对神经元有明显的剂量-效应关系.Objective To elucidate the potential damage of different doses of gamma knife irradiation to the trigeminal nerve tissues in rats, and to provide theoretical basis for the optimal strategy to treat trigeminal neuralgia pain with gamma knife.Methods Totally 65 adult male SD rats were irradiated with different doses of 20, 40, 80 and 160 Gy targeting to the trigeminal nerve, and the behaviors of the rats were observed at 1 , 2 and 4 weeks post-irradiation.To observe the conditions of cell morphological changes, autophagy, DNA damage and repair, the brain tissues were sectioned after fixing and embedding, followed by staining with HE, Nissl dyes, and immunohistochemical staining was used for MAPLC3B, 53BP1 and XRCC1 molecules.Results All the rats survived.There was neither behavioral nor cytology abnormality in 20 and 40 Gy groups.In both 80 and 160 Gy groups at 4 weeks post-irradiation, hair loss took place in the irradiated part and the surrounding area, forming the alopecic spots on the rats＇ head.HE, Nissl, LC3B, 53BP1 and XRCC1 staining showed that neuron cell shrinkage, expansion and other obvious changes occurred in 80 and 160 Gy groups,and the incidence of autophagy and DNA damage were detectable, especially at 160 Gy.DNA repair in neuron cells was obvious at 40 Gy.Conclusions Gamma knife irradiation to the trigeminal nerve showed a significant dose-effect relationship.

关 键 词：伽玛刀 放射性损伤 三叉神经 

分 类 号：R814.2[医药卫生—影像医学与核医学]