Oleosin-bFGF融合基因转化红花  

Oleosin-bFGF fusion gene transformation and expression in safflower

作　　者：薛萍[1,2] 付宏岐[1] 史俊卿[1] 庞实锋[3] 李海燕[2] 姜潮[2] 李校堃[2] 

机构地区：[1]渭南职业技术学院,陕西渭南714000 [2]吉林农业大学生物反应器与药物开发教育部工程研究中心,吉林长春130118 [3]广东医学院生物教研室,广东湛江523808

出　　处：《中国油料作物学报》2015年第6期759-765,共7页Chinese Journal of Oil Crop Sciences

基　　金：国家863计划(2011AA100606);渭南市科技创新扶持项目(2013KYJ-4);渭南职业技术学院院级重点课题(WZYZ201306)

摘　　要：利用油体蛋白融合技术,为实现oleosin-b FGF油体蛋白在转基因红花中表达奠定基础,通过农杆菌介导法将含有Ca MV35S启动子和oleosin-b FGF融合基因的T-DNA转入到红花基因组中,对潮霉素抗性红花植株的基因组进行PCR检测,提取抗性植株叶片总蛋白进行SDS-PAGE分析和Western blotting检测。PCR检测结果表明,目的基因已经转入到红花基因组,蛋白检测结果证明oleosin-b FGF融合蛋白在转基因红花叶片中有表达,但融合蛋白不稳定,发生降解。Oleosin- fusion technology was used to express oleosin- basic fibroblast growth factor（ b FGF） in transgenic safflower. T- DNA region which contained Ca MV35 S promoter and oleosin- b FGF fusion gene were transformed into safflower genome by Agrobacterium tumefaciens mediated method. Regeneration of resistant plants genomic DNA were identified by PCR,and total protein from transgenic safflower leaves were detected by SDS-PAGE and Western blot. Results showed that oleosin- b FGF fusion gene had been transformed into safflower genomic DNA,and oleosin- b FGF fusion protein were expressed in transgenic safflower leaves,but the fusion protein was unstable and degraded.

关 键 词：转基因红花 生物反应器 碱性成纤维细胞生长因子(b FGF) 油体蛋白 

分 类 号：S565.9[农业科学—作物学]