哈茨木霉复合种内3个中国新记录种的分离和鉴定  

Isolation and identification of three Chinese new records in Trichoderma harzianum species complex

作　　者：张广志[1] 张新建[1] 陈泉[1] 李哲[1] 郭凯[1] 杨合同[1] 

机构地区：[1]山东省科学院生态研究所山东省应用微生物重点实验室,山东济南250014

出　　处：《山东科学》2015年第6期43-46,51,共5页Shandong Science

基　　金：山东省科技发展计划(2014GSF121028;2013GNC20119;2012GNC11004);国家自然科学基金面上项目(31572044);国家科技基础性工作专项(2014FY120900)

摘　　要：采用THSM选择性培养基,从西藏、山东和云南等地的土壤中分离获得木霉菌株Z19、CX58-4和CY358-5。通过形态学特征、ITS r DNA和TEF-1α序列对菌株进行鉴定。结果表明,3个菌株属于哈茨木霉(Trichoderma harzianum)复合种,分别是非洲哈茨木霉(T.afroharzianum)、深褐木霉(T.atrobrunneum)和西蒙斯木霉(T.simmonsii),均为木霉属中国新记录种。We isolated three Trichoderma strains, Z19, CX58-4 and CY358-5, from soils of Tibet, Shandong and Yunnan by selective medium THSM. These strains were identified by ITS rDNA and TEF-1α sequences and morphological characteristics. Results show that the three Trichoderma strains were Trichoderma harzianum species complex, T. afroharzianum, T. atrobrunneum and T. simmonsfi. These three species are all new record to China.

关 键 词：非洲哈茨木霉 深褐木霉 西蒙斯木霉 中国新记录种 

分 类 号：Q949.32[生物学—植物学]