添加结球甘蓝4号染色体对不同基因型大白菜抽薹及BrFLCs基因表达的影响  

Effects of the Added Cabbage Chromosome 4 on Bolting Time and BrFLCs Expression of Chinese Cabbage

作　　者：顾爱侠[1] 张雅琨[1] 赵建军[1] 王彦华[1] 陈雪平[1] 申书兴[1] 

机构地区：[1]河北农业大学园艺学院,河北省蔬菜种质资源创新与利用重点实验室,河北保定071001

出　　处：《园艺学报》2015年第12期2405-2411,共7页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(31101552、31171964);河北省自然科学基金项目(C2014204093);高等学校博士学科点专项科研基金项目(20101302120006);‘十二五’农村领域国家科技计划课题(2012AA100102-5);河北省百人计划项目(2013100011);河北省高等学校科学技术研究项目(Z2010149)

摘　　要：在获得耐抽薹大白菜—结球甘蓝4号染色体单体异附加系AC4的基础上,以其为母本分别与不同基因型的2个耐抽薹大白菜自交系和2个易抽薹自交系杂交,利用根尖染色体计数法结合结球甘蓝4号染色体相对于大白菜的特异分子标记鉴定,对杂交后代进行筛选,在每个杂交组合后代中分别获得了2个2n=21和2个2n=20植株。抽薹现蕾时间鉴定结果表明每个杂交组合后代中2n=21的植株均较2n=20的植株抽薹现蕾时间推迟。对杂交后代及其父本植株中Br FLCs的表达进行了分析,结果表明Br FLCs基因表达量不是影响大白菜抽薹现蕾的唯一主要因素,基因组背景对抽薹现蕾时间效应大于Br FLCs基因,同时外源结球甘蓝4号染色体的添加对Br FLCs基因表达的效应受大白菜基因组背景的影响。Monosomic addition line of Chinese cabbage introduced alien cabbage chromosome 4 had been obtained. In this research,the hybridizations between Chinese cabbage monosomic alien addition line and four Chinese cabbage inbred lines with early or late bolting were developed. These hybrids were identified by chromosome counting of root tip and chromosome 4 specific markers of cabbage compared with Chinese cabbage. Two 2n = 21 and two 2n = 20 plants were obtained in every cross combination. The results of bolting investigation for hybrids indicated that the bolting of plants with chromosome 2n = 21 were later than the plants with 2n = 20 from the same hybridized combination. The gene Br FLCs expression in hybrids and male parents were analyzed by the Real-time PCR. The results showed that the relative expression of Br FLCs was not the only major factor,the effect of genome on bolting and budding was more than that only from Br FLCs. The effect of added cabbage chromosome 4 on the Br FLCs expression was influenced by the background of Chinese cabbage genome.

关 键 词：大白菜 结球甘蓝 单体异附加系 抽薹 Br FLCs 

分 类 号：S634.1[农业科学—蔬菜学]