金叶弯刺蔷薇×山刺玫遗传连锁图谱的初步构建  

Primary Construction of a Genetic Linkage Map of Rosa beggeriana‘Aurea'× R. Davurica

作　　者：杨树华[1] 李世超[1] 贾瑞冬[1] 赵鑫[1] 金茂勇 李凤杰 葛红[1] 

机构地区：[1]中国农业科学院蔬菜花卉研究所,北京100081 [2]北京市大兴区园林绿化局,北京102600

出　　处：《园艺学报》2015年第12期2526-2534,共9页Acta Horticulturae Sinica

基　　金：国家‘863’计划项目(2011AA100208);农业部‘948’项目(2011-G17);国家科技支撑计划项目(2012BAD01B0704);北京市大兴区与中国农业科学院科技合作项目;农业部园艺作物生物学与种质创制重点实验室项目

摘　　要：以金叶弯刺蔷薇(Rosa beggeriana‘Aurea’,叶色突变材料)和山刺玫(R.davurica)为杂交亲本建立F1群体,利用AFLP和SSR分子标记进行遗传连锁图谱构建。结果表明:124个AFLP标记、25个SSR标记及1个形态标记定位于父、母本遗传连锁图谱的13个连锁群上,其中92个分子标记定位于金叶弯刺蔷薇7个连锁群,遗传距离覆盖度为607.2 c M,金叶突变性状被定位于第4连锁群;57个分子标记定位于山刺玫6个连锁群,遗传距离覆盖度为437.1 c M。两类分子标记的总作图效率达到69.6%。发生偏分离的分子标记数量为44个,偏分离率为29.5%。The F1 population of Rosa beggeriana‘Aurea＇× R. davurica was established for the construction of genetic linkage map using AFLP and SSR markers. The results showed that 124 AFLP markers,25 SSR markers and 1 morphological marker were located in total 13 linkage groups of the parents. There were 92 markers covered the genetic distances of 607.2 c M in 7 linkage groups of R. beggeriana‘Aurea＇,which the yellow-leaf mutant trait was located in the 4th linkage group. In addition,there were 57 markers covered the genetic distances of 437.1 c M in 6 linkage groups of R. davurica. The total mapping efficiency reached 69.6% and 44 distorted markers were detected with the distortion rate of 29.5%.

关 键 词：金叶弯刺蔷薇 山刺玫 遗传作图 AFLP标记 SSR标记 

分 类 号：S685.12[农业科学—观赏园艺]