^(137)Cs γ射线辐照对小鼠造血及免疫功能的影响  

Effects of ^(137)Cs Gamma Irradiation on The Hematopoietic and Immune Function in Mice

作　　者：张丽娟[1] 路璐[1] 王月英[1] 李德冠[1] 张俊伶[1] 孟爱民[1] 马蕊[1] 

机构地区：[1]中国医学科学院北京协和医学院放射医学研究所天津市分子核医学重点实验室,天津300192

出　　处：《中国辐射卫生》2015年第3期196-198,共3页Chinese Journal of Radiological Health

基　　金：国家自然科学基金(81372928);天津市重点基金(15JCZDJC35200);放射所开发基金(1539)

摘　　要：目的观察射线辐照对IRM-2、ICR、615、C57BL/6J四种小鼠造血及免疫功能的影响。方法用137Csγ射线对四种小鼠进行4.0 Gy照射后,分别对四种小鼠的外周血分类、骨髓有核细胞及脏器指数进行测定,并检测小鼠腹腔巨噬细胞吞噬鸡红细胞的功能。结果 IRM-2小鼠白细胞总数、骨髓有核细胞及巨噬细胞吞噬率均高于615、ICR、C57BL/6J小鼠,IRM-2小鼠与615、ICR、C57BL/6J小鼠比较,差异具有统计学意义(P<0.01)。四种小鼠的白细胞分类基本一致。照射后第4周,IRM-2小鼠的WBC、BMNC数量与ICR、615、C57BL/6J小鼠比较差异有统计学意义(P<0.01)。结论 IRM-2小鼠免疫及造血功能较强,是较好的应用于实验研究的动物模型。Objective To observe the effect of irradiation on the hematopoietic and immune function of four kinds of mice,IRM- 2、ICR、615 and C57 BL /6J. Methods After the 4. 0Gy radiation with137 Cs gamma ray,respectively peripheral blood classification was performed,bone marrow nucleated cells（ BMNC） count and visceral index of the four kinds of mice were measured and the phagocytic function of peritoneal macrophages on chicken red blood cells were tested. Results The total number of WBC,the number of BMNC and macrophage phagocytosis rate of IRM- 2 are higher than those of other strains,and the differences between IRM- 2 and 615 or ICR or C57 BL /6J were statistically significant（ P〈0. 01）. The classification of the four kinds of mice white blood cells is basically consistent. Fourth weeks after irradiation,the differences in the number of WBC and BMNC in IRM- 2 were statistically significant compared with 615 or ICR or C57 BL /6J（ P〈0. 01）. Conclusion IRM- 2 mice have better hematopoietic and immune functions,and are good animal model applied in the experimental study.

关 键 词：小鼠 辐射损伤 造血功能 免疫功能 模型 

分 类 号：Q345.1[生物学—遗传学]